Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LMH63_RS16695 Genome accession   NZ_CP086615
Coordinates   3569438..3570436 (+) Length   332 a.a.
NCBI ID   WP_109678602.1    Uniprot ID   A0A2U2N215
Organism   Spiribacter halobius strain E85     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3564438..3575436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS16675 (LMH63_16670) coaD 3565249..3565743 (-) 495 WP_109678598.1 pantetheine-phosphate adenylyltransferase -
  LMH63_RS16680 (LMH63_16675) rsmD 3565904..3566461 (-) 558 WP_109678599.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  LMH63_RS16685 (LMH63_16680) - 3566463..3567788 (-) 1326 WP_109678600.1 pitrilysin family protein -
  LMH63_RS16690 (LMH63_16685) - 3567785..3569299 (-) 1515 WP_109678601.1 pitrilysin family protein -
  LMH63_RS16695 (LMH63_16690) pilA 3569438..3570436 (+) 999 WP_109678602.1 signal recognition particle-docking protein FtsY Machinery gene
  LMH63_RS16700 (LMH63_16695) ftsE 3570433..3571101 (+) 669 WP_109678603.1 cell division ATP-binding protein FtsE -
  LMH63_RS16705 (LMH63_16700) ftsX 3571103..3572047 (+) 945 WP_109678604.1 permease-like cell division protein FtsX -
  LMH63_RS16710 (LMH63_16705) rpoH 3572545..3573426 (+) 882 WP_109678605.1 RNA polymerase sigma factor RpoH -
  LMH63_RS16715 (LMH63_16710) radC 3573635..3574309 (-) 675 WP_109678606.1 DNA repair protein RadC -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35326.20 Da        Isoelectric Point: 6.1760

>NTDB_id=625724 LMH63_RS16695 WP_109678602.1 3569438..3570436(+) (pilA) [Spiribacter halobius strain E85]
MLPFRRKRSGDDGADTPTREGRRPGLVARLRAGLRRTGSGLSGLFLGRKQIDEDLLEELETLLLTADVGVEATRRIIDDL
TARLRRNELADTGALHQALREDMLAILAPVAQPLEIPPDKRPFVLLTVGVNGAGKTTTIGKLAARLRDQGHGVILAAGDT
FRAAAVEQLRAWGERTGTPVISQPTGADAASVVYDAHQAARARGLDVLIADTAGRLHTQGNLMEELRKIHRVLGKQDPDS
PHEVLLVVDAGTGQNALAQARQFHESIGLTGLAITKLDGTAKGGVIFAIAQQLGIPIRFIGVGEQVEDLRPFDAEAFVDA
LLAGATGADDGE

Nucleotide


Download         Length: 999 bp        

>NTDB_id=625724 LMH63_RS16695 WP_109678602.1 3569438..3570436(+) (pilA) [Spiribacter halobius strain E85]
ATGCTGCCTTTCCGCCGCAAGCGCTCCGGCGACGACGGAGCCGACACTCCGACCCGCGAGGGCCGGCGTCCGGGGCTCGT
GGCCCGCCTGCGGGCGGGGCTCCGTCGCACCGGCAGCGGCCTGTCCGGCCTGTTCCTCGGTCGCAAGCAGATCGACGAGG
ACCTGCTCGAGGAGCTGGAGACGCTGCTGCTGACGGCGGACGTGGGCGTCGAGGCCACCCGCCGCATCATCGACGACCTC
ACCGCCCGCCTGCGCCGCAACGAGCTCGCCGACACCGGCGCGCTGCACCAGGCGCTGCGGGAAGACATGCTTGCGATCCT
GGCGCCGGTGGCACAGCCGCTGGAGATCCCACCGGATAAGCGCCCCTTCGTGCTGCTCACCGTCGGCGTCAACGGCGCCG
GCAAGACCACGACCATCGGCAAGCTCGCCGCGCGCCTGCGCGACCAGGGCCATGGCGTGATCCTCGCCGCCGGCGACACC
TTCCGCGCGGCGGCGGTGGAGCAGCTGCGGGCCTGGGGCGAGCGCACCGGCACGCCGGTGATCAGCCAGCCCACCGGCGC
CGATGCCGCCTCCGTGGTGTACGACGCGCACCAGGCCGCCCGCGCCCGCGGGCTGGACGTGCTGATTGCGGACACCGCCG
GGCGCCTGCATACCCAGGGCAACCTGATGGAGGAGCTGCGCAAGATCCACCGCGTGCTCGGCAAGCAGGATCCGGACTCG
CCGCACGAGGTGCTGCTGGTGGTGGACGCCGGCACGGGCCAGAACGCGCTGGCCCAGGCGCGGCAGTTCCACGAGTCCAT
CGGCCTCACCGGCCTGGCCATCACCAAGCTCGACGGCACCGCCAAGGGCGGCGTGATCTTCGCCATCGCCCAGCAGCTCG
GCATCCCCATCCGTTTCATCGGCGTCGGCGAGCAGGTGGAGGACCTGCGCCCCTTCGACGCCGAGGCTTTCGTCGACGCG
CTGCTGGCCGGCGCCACCGGAGCCGACGACGGCGAATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(123-322)

SRP54-type protein, helical bundle domain (SRP54_N)

(38-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2N215

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.722

93.072

0.5