Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LMH63_RS04120 Genome accession   NZ_CP086615
Coordinates   902445..903917 (-) Length   490 a.a.
NCBI ID   WP_109676008.1    Uniprot ID   A0A2U2N7P5
Organism   Spiribacter halobius strain E85     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 897445..908917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS04090 (LMH63_04090) - 899188..899475 (-) 288 WP_109676019.1 HPr family phosphocarrier protein -
  LMH63_RS04095 (LMH63_04095) - 899465..899884 (-) 420 WP_109676017.1 PTS sugar transporter subunit IIA -
  LMH63_RS04100 (LMH63_04100) rapZ 899881..900738 (-) 858 WP_109676015.1 RNase adapter RapZ -
  LMH63_RS04105 (LMH63_04105) - 900742..901560 (-) 819 WP_109676013.1 hypothetical protein -
  LMH63_RS04110 (LMH63_04110) ptsN 901557..902027 (-) 471 WP_109676012.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LMH63_RS04115 (LMH63_04115) hpf 902098..902424 (-) 327 WP_109676010.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LMH63_RS04120 (LMH63_04120) rpoN/rpoN1 902445..903917 (-) 1473 WP_109676008.1 RNA polymerase factor sigma-54 Machinery gene
  LMH63_RS04125 (LMH63_04125) lptB 904065..904805 (-) 741 WP_199225573.1 LPS export ABC transporter ATP-binding protein -
  LMH63_RS04130 (LMH63_04130) lptA 904802..905320 (-) 519 WP_109676006.1 lipopolysaccharide transport periplasmic protein LptA -
  LMH63_RS04135 (LMH63_04135) lptC 905304..905870 (-) 567 WP_109676004.1 LPS export ABC transporter periplasmic protein LptC -
  LMH63_RS04140 (LMH63_04140) - 905863..906423 (-) 561 WP_109676002.1 HAD family hydrolase -
  LMH63_RS04145 (LMH63_04145) - 906423..907427 (-) 1005 WP_109676000.1 KpsF/GutQ family sugar-phosphate isomerase -
  LMH63_RS04150 (LMH63_04150) - 907445..908413 (-) 969 WP_109675998.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 490 a.a.        Molecular weight: 55248.01 Da        Isoelectric Point: 4.4339

>NTDB_id=625711 LMH63_RS04120 WP_109676008.1 902445..903917(-) (rpoN/rpoN1) [Spiribacter halobius strain E85]
MKQSLQLRLGQQLTMTPQLQQAIRLLQLPTLELRMEIQQALESNLMLEAAEEAELEAAAERDEDGAEASEAAPDRTEQDL
GGEEPDAARLTEDLPVDSSWEDVYDGSTTYSHSGSDDERDLFENQSGGEESLRDYLIWQLQMAPISPRDMAIAEAIVDAV
RDDGYLGEPLEEIGAGLPPELEVEPDDLQAVLARVQRLDPVGVAARDVREALLVQLDQLDPATPHLAAARELVDQHLELL
VSRQFAQLKRRMRLDLETLQAALALVQTLDPRPGARFSTERTEYVVPDVFVRRVNGDWQVEVNPDACPRLRVNSYYASLV
RRADNSRDNSLMRQHLQEARWLIKSLRSRNDTLLKVAECIVERQRGFLEQGEEAMQPLVLREVAETIEMHESTVSRITNQ
KYMHTPRGTFEFKYFFSSHVQTVDGGECSATAIRARIRRLVADEDPASPLSDSRLAEILREEGINVARRTVAKYREAMAI
ASSTERKRLA

Nucleotide


Download         Length: 1473 bp        

>NTDB_id=625711 LMH63_RS04120 WP_109676008.1 902445..903917(-) (rpoN/rpoN1) [Spiribacter halobius strain E85]
ATGAAGCAGTCGCTACAGCTCCGACTCGGGCAACAGCTCACCATGACCCCGCAGCTGCAGCAGGCCATCCGGCTGCTGCA
GCTGCCGACCCTCGAACTGCGCATGGAGATCCAGCAGGCGCTGGAGTCCAACCTGATGCTCGAGGCCGCGGAGGAAGCGG
AGCTCGAGGCGGCCGCGGAGCGCGACGAGGACGGGGCCGAGGCGTCGGAGGCCGCACCGGACCGCACGGAACAGGATCTC
GGCGGCGAGGAGCCGGATGCCGCGCGCCTCACCGAGGACCTCCCGGTGGACTCCAGCTGGGAGGACGTCTACGACGGCAG
CACCACCTACAGCCACTCCGGTTCCGACGACGAGCGCGACCTCTTCGAGAACCAGTCCGGCGGCGAGGAATCCCTGCGCG
ACTACCTCATCTGGCAGCTGCAGATGGCTCCGATCTCGCCCCGGGACATGGCCATCGCCGAGGCCATCGTCGACGCCGTG
CGCGACGACGGCTACCTCGGCGAGCCGCTGGAGGAGATCGGCGCCGGCCTGCCGCCGGAGCTGGAGGTGGAGCCGGACGA
CCTGCAGGCGGTGCTGGCCCGGGTGCAGCGGCTGGACCCGGTGGGTGTGGCGGCCCGGGACGTGCGCGAGGCGCTGCTGG
TGCAGCTGGACCAGCTGGACCCGGCCACGCCCCATCTGGCGGCGGCCCGCGAGCTCGTGGACCAGCACCTGGAGCTGCTG
GTCAGCCGCCAGTTCGCCCAGCTCAAGCGCCGCATGCGGCTCGACCTCGAGACCCTGCAGGCGGCCCTGGCGCTGGTGCA
GACCCTGGACCCCCGCCCCGGCGCGCGCTTCAGCACCGAGCGCACCGAGTACGTGGTGCCGGACGTGTTCGTGCGCCGCG
TCAACGGCGACTGGCAGGTGGAGGTGAACCCGGACGCCTGCCCGCGGCTGCGGGTGAACAGCTACTACGCCAGCCTGGTG
CGCCGGGCCGACAACAGCCGCGACAACAGCCTCATGCGCCAGCACCTGCAGGAGGCGCGATGGCTGATCAAGAGCCTGCG
ATCGCGCAACGACACCCTGCTCAAGGTGGCGGAGTGCATCGTCGAGCGCCAGCGGGGCTTCCTCGAGCAGGGCGAGGAGG
CGATGCAGCCGCTGGTGCTGCGCGAGGTGGCCGAAACCATCGAGATGCACGAGTCCACGGTCTCGCGCATCACCAACCAG
AAGTACATGCACACCCCGCGCGGGACCTTCGAGTTCAAGTACTTCTTCTCCAGTCACGTGCAGACCGTGGACGGCGGCGA
ATGCTCCGCCACGGCGATTCGCGCGCGCATCCGGCGCCTGGTCGCCGACGAGGATCCGGCGAGCCCGCTGAGCGACTCGC
GGCTCGCCGAGATCCTGCGCGAAGAGGGCATCAACGTGGCACGCAGGACGGTGGCCAAGTACCGAGAGGCCATGGCCATC
GCCTCGTCCACCGAACGCAAGCGGCTGGCCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-488)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(123-316)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2N7P5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.184

100

0.482