Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   LK341_RS09295 Genome accession   NZ_CP086570
Coordinates   1952433..1954061 (+) Length   542 a.a.
NCBI ID   WP_250327249.1    Uniprot ID   -
Organism   Staphylococcus argenteus strain RIVM_M020832     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 1945902..1960826 1952433..1954061 within 0


Gene organization within MGE regions


Location: 1945902..1960826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK341_RS09275 (LK341_09265) - 1947506..1948552 (+) 1047 WP_001042025.1 hypothetical protein -
  LK341_RS09280 (LK341_09270) - 1948745..1949041 (+) 297 WP_000273825.1 single-stranded DNA-binding protein -
  LK341_RS09285 (LK341_09275) - 1949041..1950828 (+) 1788 WP_000122624.1 DUF927 domain-containing protein -
  LK341_RS09290 (LK341_09280) ccrA 1951062..1952411 (+) 1350 WP_000815651.1 recombinase family protein Machinery gene
  LK341_RS09295 (LK341_09285) ccrB 1952433..1954061 (+) 1629 WP_250327249.1 recombinase family protein Machinery gene
  LK341_RS09300 (LK341_09290) - 1954583..1954933 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LK341_RS09305 (LK341_09295) - 1955020..1955331 (+) 312 WP_000700851.1 DUF960 family protein -
  LK341_RS09310 (LK341_09300) - 1955346..1955852 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  LK341_RS09315 (LK341_09305) - 1955904..1957511 (+) 1608 Protein_1798 IS1182 family transposase -
  LK341_RS09320 (LK341_09310) - 1957502..1957762 (-) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  LK341_RS09325 (LK341_09315) - 1957734..1958720 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  LK341_RS09330 (LK341_09320) mecA 1958817..1960826 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62668.86 Da        Isoelectric Point: 9.7970

>NTDB_id=625506 LK341_RS09295 WP_250327249.1 1952433..1954061(+) (ccrB) [Staphylococcus argenteus strain RIVM_M020832]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEINTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=625506 LK341_RS09295 WP_250327249.1 1952433..1954061(+) (ccrB) [Staphylococcus argenteus strain RIVM_M020832]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAATCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAACGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTGCTATGCCAAAGGACATGGCTATCGTAAAATTGCCAATGCATTGAATCACAAAGGATATGTCACTAAAA
AGGGGAAACCTTTCAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.708

100

0.987