Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LK341_RS02260 Genome accession   NZ_CP086570
Coordinates   432696..433670 (+) Length   324 a.a.
NCBI ID   WP_250327261.1    Uniprot ID   -
Organism   Staphylococcus argenteus strain RIVM_M020832     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 427696..438670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK341_RS02225 (LK341_02225) rpmG 428153..428302 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  LK341_RS02230 (LK341_02230) - 428514..429053 (+) 540 WP_072324191.1 5-formyltetrahydrofolate cyclo-ligase -
  LK341_RS02235 (LK341_02235) - 429065..430528 (+) 1464 WP_031787264.1 rhomboid family intramembrane serine protease -
  LK341_RS02240 (LK341_02240) - 430509..430709 (+) 201 WP_000087563.1 YqgQ family protein -
  LK341_RS02245 (LK341_02245) - 430709..431695 (+) 987 WP_000161316.1 ROK family glucokinase -
  LK341_RS02250 (LK341_02250) - 431695..432024 (+) 330 WP_001018874.1 MTH1187 family thiamine-binding protein -
  LK341_RS02255 (LK341_02255) - 432021..432644 (+) 624 WP_031787263.1 MBL fold metallo-hydrolase -
  LK341_RS02260 (LK341_02260) comGA 432696..433670 (+) 975 WP_250327261.1 competence type IV pilus ATPase ComGA Machinery gene
  LK341_RS02265 (LK341_02265) comGB 433642..434712 (+) 1071 WP_031787261.1 competence type IV pilus assembly protein ComGB Machinery gene
  LK341_RS02270 (LK341_02270) comGC 434727..435038 (+) 312 WP_000921699.1 competence type IV pilus major pilin ComGC Machinery gene
  LK341_RS02275 (LK341_02275) comGD 435016..435462 (+) 447 WP_072324131.1 competence type IV pilus minor pilin ComGD Machinery gene
  LK341_RS02280 (LK341_02280) comGE 435449..435748 (+) 300 WP_031787259.1 hypothetical protein Machinery gene
  LK341_RS02285 (LK341_02285) comGF 435708..436163 (+) 456 WP_078097699.1 competence type IV pilus minor pilin ComGF Machinery gene
  LK341_RS02290 (LK341_02290) - 436262..436411 (+) 150 WP_072324192.1 hypothetical protein -
  LK341_RS02295 (LK341_02295) - 436398..436922 (+) 525 WP_031787256.1 shikimate kinase -
  LK341_RS02300 (LK341_02300) gcvT 437079..438170 (+) 1092 WP_000093342.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36867.03 Da        Isoelectric Point: 9.0234

>NTDB_id=625482 LK341_RS02260 WP_250327261.1 432696..433670(+) (comGA) [Staphylococcus argenteus strain RIVM_M020832]
MKILFQEIINKAIVMKASDVHFIPVHNEVSIKFRINDNLEQYEEIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQYFKQTKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNEKAGINYVNSFKAILRCDPDVILIGEIRDKEVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGLSVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLQYFFSHNHSLPTSFKKLETKLDDMTKAGVICESTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=625482 LK341_RS02260 WP_250327261.1 432696..433670(+) (comGA) [Staphylococcus argenteus strain RIVM_M020832]
ATGAAGATATTATTTCAAGAGATAATCAACAAAGCGATAGTAATGAAAGCGAGTGATGTCCATTTTATTCCTGTTCATAA
TGAAGTGAGTATTAAATTTCGTATAAATGACAATTTGGAACAGTATGAAGAAATTGGAAATAGTATTTATCAAAAGCTAT
TAGTTTATATGAAATTTCAAGCTGGTCTCGATGTATCTACGCAACAAGTTGCTCAGAGTGGTCGTTATAGCTACCATTTT
AATAAAATTTATTTTTTAAGAATTTCAACATTGCCATTATCACTTGGTCAAGAAAGTTGTGTGATAAGAATAGTTCCTCA
ATATTTTAAACAAACTAAATCAACATATAAATTTAATGATTTTAAACATTTAATGAATAAGAAACAAGGCTTATTGTTAT
TTAGTGGTCCGACGGGTTCAGGTAAAAGTACTTTAATGTATCAAATGGTCTCATACGCTAACAAAGCGTTAAACTTAAAT
GTTATATCTATTGAAGACCCAGTAGAAATGCAAATTCCTGGAATAGTTCAAATAAACGTAAATGAAAAAGCTGGGATTAA
CTATGTCAATTCATTTAAAGCGATATTAAGGTGTGACCCAGATGTTATTCTTATAGGGGAAATTAGAGATAAAGAAGTAG
CTAAATGTGTCATCCAAGCAAGTTTAAGTGGTCATCTAGTACTCACTACCTTACATGCAACAGATTGTAAAGGTGCAATT
TTAAGATTACTAGAAATGGGGCTTTCAGTACAAGAACTAATACAAGCAACTAATTTAATTATAAATCAAAGACTTGTTAC
TACAATTAAACAGCAACGACAATTAGTGTGTGAAATTTTATCACAACAACAACTTCAGTATTTCTTCTCGCATAATCATT
CATTACCTACCTCATTTAAAAAACTAGAAACAAAGCTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAAGTACAATG
GATAAGTATATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

95.679

100

0.957
  comGA Staphylococcus aureus N315

95.679

100

0.957