Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYO15_RS18830 Genome accession   NZ_CP103758
Coordinates   3841021..3841461 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain p10B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3836021..3846461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYO15_RS18810 (NYO15_18810) aroP 3837019..3838392 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NYO15_RS18815 (NYO15_18815) ampE 3838435..3839289 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NYO15_RS18820 (NYO15_18820) ampD 3839286..3839837 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NYO15_RS18825 (NYO15_18825) nadC 3839925..3840818 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NYO15_RS18830 (NYO15_18830) pilA 3841021..3841461 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NYO15_RS18835 (NYO15_18835) pilB 3841471..3842856 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NYO15_RS18840 (NYO15_18840) hofC 3842846..3844048 (+) 1203 WP_000157284.1 protein transport protein HofC -
  NYO15_RS18845 (NYO15_18845) guaC 3844083..3845126 (-) 1044 WP_001217338.1 GMP reductase -
  NYO15_RS18850 (NYO15_18850) - 3845282..3845326 (-) 45 WP_120795372.1 protein YacM -
  NYO15_RS18855 (NYO15_18855) coaE 3845351..3845971 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=624978 NYO15_RS18830 WP_000360904.1 3841021..3841461(+) (pilA) [Escherichia coli strain p10B]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=624978 NYO15_RS18830 WP_000360904.1 3841021..3841461(+) (pilA) [Escherichia coli strain p10B]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363