Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NYO15_RS02470 Genome accession   NZ_CP103758
Coordinates   511928..513361 (-) Length   477 a.a.
NCBI ID   WP_000809040.1    Uniprot ID   -
Organism   Escherichia coli strain p10B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506928..518361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYO15_RS02435 (NYO15_02435) elbB 507619..508272 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  NYO15_RS02440 (NYO15_02440) mtgA 508269..508997 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NYO15_RS02445 (NYO15_02445) yrbL 508994..509626 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  NYO15_RS02450 (NYO15_02450) npr 509840..510112 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  NYO15_RS02455 (NYO15_02455) rapZ 510109..510963 (-) 855 WP_000243741.1 RNase adapter RapZ -
  NYO15_RS02460 (NYO15_02460) ptsN 511009..511500 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NYO15_RS02465 (NYO15_02465) hpf 511618..511905 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  NYO15_RS02470 (NYO15_02470) rpoN/rpoN1 511928..513361 (-) 1434 WP_000809040.1 RNA polymerase factor sigma-54 Machinery gene
  NYO15_RS02475 (NYO15_02475) lptB 513409..514134 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  NYO15_RS02480 (NYO15_02480) lptA 514141..514698 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NYO15_RS02485 (NYO15_02485) lptC 514667..515242 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  NYO15_RS02490 (NYO15_02490) kdsC 515239..515805 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NYO15_RS02495 (NYO15_02495) kdsD 515826..516812 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  NYO15_RS02500 (NYO15_02500) yrbG 516826..517803 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53975.76 Da        Isoelectric Point: 4.3639

>NTDB_id=624933 NYO15_RS02470 WP_000809040.1 511928..513361(-) (rpoN/rpoN1) [Escherichia coli strain p10B]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=624933 NYO15_RS02470 WP_000809040.1 511928..513361(-) (rpoN/rpoN1) [Escherichia coli strain p10B]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTTCAACAGGCGATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465