Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5T33_RS04840 Genome accession   NZ_CP103739
Coordinates   994380..994820 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 3359     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 989380..999820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T33_RS04815 (M5T33_04815) coaE 989870..990490 (-) 621 WP_001269532.1 dephospho-CoA kinase -
  M5T33_RS04820 (M5T33_04820) - 990515..990559 (+) 45 WP_120795372.1 protein YacM -
  M5T33_RS04825 (M5T33_04825) guaC 990715..991758 (+) 1044 WP_001217338.1 GMP reductase -
  M5T33_RS04830 (M5T33_04830) hofC 991793..992995 (-) 1203 WP_000157266.1 protein transport protein HofC -
  M5T33_RS04835 (M5T33_04835) pilB 992985..994370 (-) 1386 WP_001025147.1 type II secretion system protein GspE Machinery gene
  M5T33_RS04840 (M5T33_04840) pilA 994380..994820 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  M5T33_RS04845 (M5T33_04845) nadC 995023..995916 (-) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5T33_RS04850 (M5T33_04850) ampD 996004..996555 (+) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5T33_RS04855 (M5T33_04855) ampE 996552..997406 (+) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  M5T33_RS04860 (M5T33_04860) aroP 997449..998822 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=624609 M5T33_RS04840 WP_000360904.1 994380..994820(-) (pilA) [Escherichia coli strain 3359]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=624609 M5T33_RS04840 WP_000360904.1 994380..994820(-) (pilA) [Escherichia coli strain 3359]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363