Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KT882_RS04445 Genome accession   NZ_CP086370
Coordinates   1002656..1004140 (+) Length   494 a.a.
NCBI ID   WP_161071377.1    Uniprot ID   A0A7X4H973
Organism   Pseudoduganella aquatica strain HC52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 997656..1009140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KT882_RS04420 - 999121..1000119 (+) 999 WP_161071380.1 SIS domain-containing protein -
  KT882_RS04425 - 1000121..1000660 (+) 540 WP_161071379.1 HAD family hydrolase -
  KT882_RS04430 lptC 1000672..1001265 (+) 594 WP_228894572.1 LPS export ABC transporter periplasmic protein LptC -
  KT882_RS04435 lptA 1001280..1001876 (+) 597 WP_228894576.1 lipopolysaccharide transport periplasmic protein LptA -
  KT882_RS04440 lptB 1001879..1002640 (+) 762 WP_228894577.1 LPS export ABC transporter ATP-binding protein -
  KT882_RS04445 rpoN/rpoN1 1002656..1004140 (+) 1485 WP_161071377.1 RNA polymerase factor sigma-54 Machinery gene
  KT882_RS04450 hpf 1004210..1004566 (+) 357 WP_161071376.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KT882_RS04455 - 1004662..1005741 (+) 1080 WP_228894579.1 enoyl-CoA hydratase/isomerase family protein -
  KT882_RS04460 rapZ 1005742..1006605 (-) 864 WP_161071374.1 RNase adapter RapZ -
  KT882_RS04465 hprK 1006677..1007612 (-) 936 WP_056404135.1 HPr(Ser) kinase/phosphatase -
  KT882_RS04470 - 1007656..1008138 (-) 483 WP_107870025.1 PTS sugar transporter subunit IIA -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 54598.05 Da        Isoelectric Point: 4.9164

>NTDB_id=624552 KT882_RS04445 WP_161071377.1 1002656..1004140(+) (rpoN/rpoN1) [Pseudoduganella aquatica strain HC52]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLGENPLLERLDDPLDRSLRLLGDGAINQTQSDGADASPAD
VGAPAQDSAPAGEGDPFDGAGGDSASGAEADTDWSDAGRSKAPDDEDSRPQLEASHCTLREHLMEQMRVTVQQPRDRVLM
ELIIDALDENGYLEEPLEEIHARLPEEADVELEELQTALTLLQSFDPIGVGARSASECLALQIKRMPGVPLVTRRMAICI
VEKHLAWFAQREFNKLKKALDCDDEDLREAQAVIRQCNPHPGAEFASDVSDYVVPDVIVKKARNGWQVSLNNDVMPRLRV
NALYANMLKQGKGESAMGAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTIS
RVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYR
EALKIPPVSLRKAL

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=624552 KT882_RS04445 WP_161071377.1 1002656..1004140(+) (rpoN/rpoN1) [Pseudoduganella aquatica strain HC52]
ATGAAACAGTCACTGCAACTCCGCACCTCTCAGCACCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGATTGCTGCA
GCTGTCCACGCTGGAGCTGCACCAGGAACTGGAACAGCTGCTGGGCGAGAACCCGCTGCTGGAGCGCCTGGATGATCCGC
TGGACCGCTCGCTGCGCCTGCTCGGCGACGGCGCCATCAACCAGACCCAGTCCGACGGCGCCGACGCGTCGCCGGCCGAC
GTGGGCGCGCCGGCGCAGGACAGCGCGCCGGCCGGCGAGGGCGACCCCTTCGACGGCGCCGGTGGCGACAGCGCCAGCGG
CGCCGAGGCCGACACCGACTGGAGCGACGCCGGCCGCAGCAAGGCGCCGGACGACGAGGACTCGCGCCCGCAGCTCGAAG
CGAGCCACTGCACCTTGCGCGAGCACCTGATGGAGCAGATGCGCGTGACCGTGCAGCAGCCGCGCGACCGCGTGCTGATG
GAGCTGATCATCGACGCGCTGGACGAGAACGGCTACCTGGAAGAGCCGCTGGAAGAGATCCATGCGCGCCTGCCGGAAGA
GGCGGACGTGGAGCTGGAGGAGCTGCAAACGGCGCTGACCCTGCTGCAAAGTTTCGACCCCATCGGCGTGGGCGCGCGCA
GCGCCTCCGAGTGCCTGGCGCTGCAGATCAAGCGCATGCCCGGCGTGCCGCTGGTCACGCGACGCATGGCCATCTGCATC
GTGGAAAAGCACCTGGCCTGGTTCGCGCAGCGCGAATTCAACAAGCTGAAAAAAGCCCTCGATTGCGACGACGAGGACCT
GCGCGAAGCGCAAGCGGTGATCCGCCAGTGCAACCCGCACCCGGGCGCCGAGTTCGCTTCCGACGTGTCCGACTACGTGG
TGCCGGACGTGATCGTGAAGAAGGCGCGCAATGGCTGGCAGGTCTCCTTGAACAACGACGTGATGCCGCGCCTGCGCGTG
AACGCGCTGTACGCCAACATGCTCAAGCAGGGAAAAGGCGAGTCGGCCATGGGCGCGCAGCTGCAGGAAGCCAAGTGGCT
GATCAAGAATATGCGCCAGCGTTTCGACACGATACTGCGCGTGGCGCAAGCGATAGTAGAAAGACAAAAGAACTTTTTCA
GCCATGGCGCGGTTGCCATGAGACCCCTTGTGTTGCGTGAAATTGCTGATACACTGGGACTACACGAGAGCACTATTTCT
CGGGTAACAACTCAGAAATACATGCTGACTCCGCATGGGATGTTTGAGTTGAAATATTTCTTTGGCAGCCACGTCGCCAC
CGAAGCAGGAGGAGAAGCATCCTCGACGGCGATACGGGCGCTGATAGTGCAACTGACAGGAGCAGAAGACCCGAAAAATC
CTTTATCCGACAGTAAGATTGCGGACATGCTGGGAGAACAGGGCATGGTAATTGCGCGACGGACTGTTGCCAAATATCGC
GAAGCCTTGAAAATCCCTCCGGTAAGTCTGCGTAAAGCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-492)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-324)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X4H973

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.198

100

0.615