Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LMD38_RS07080 Genome accession   NZ_CP086328
Coordinates   1331523..1331957 (+) Length   144 a.a.
NCBI ID   WP_000811499.1    Uniprot ID   A0A1J9ZQC5
Organism   Bacillus pacificus strain anQ-h4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1326523..1336957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMD38_RS07060 (LMD38_07060) - 1327259..1328422 (-) 1164 WP_062821082.1 aminotransferase A -
  LMD38_RS07065 (LMD38_07065) - 1328546..1328746 (-) 201 WP_000929285.1 hypothetical protein -
  LMD38_RS07070 (LMD38_07070) kinB 1329056..1330330 (+) 1275 WP_062821081.1 sporulation sensor histidine kinase KinB -
  LMD38_RS07075 (LMD38_07075) - 1330384..1331133 (-) 750 WP_000388461.1 DUF3967 domain-containing protein -
  LMD38_RS07080 (LMD38_07080) nucA/comI 1331523..1331957 (+) 435 WP_000811499.1 DNA-entry nuclease Machinery gene
  LMD38_RS07085 (LMD38_07085) comJ 1331993..1332382 (+) 390 WP_061182412.1 competence protein ComJ -
  LMD38_RS07090 (LMD38_07090) metE 1332420..1334708 (-) 2289 WP_062821080.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  LMD38_RS07095 (LMD38_07095) - 1335378..1335935 (-) 558 WP_062821079.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15996.35 Da        Isoelectric Point: 8.4331

>NTDB_id=624303 LMD38_RS07080 WP_000811499.1 1331523..1331957(+) (nucA/comI) [Bacillus pacificus strain anQ-h4]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=624303 LMD38_RS07080 WP_000811499.1 1331523..1331957(+) (nucA/comI) [Bacillus pacificus strain anQ-h4]
ATGAAACAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGATCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCGCATATTGAATATATAAGTCCAGCCGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J9ZQC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556