Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LMA00_RS14215 Genome accession   NZ_CP086304
Coordinates   3038785..3040290 (+) Length   501 a.a.
NCBI ID   WP_006752626.1    Uniprot ID   A0AA41JK91
Organism   Burkholderia ambifaria strain B21-004     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3033785..3045290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMA00_RS14190 (LMA00_14190) kdsD 3035018..3036001 (+) 984 WP_006752621.1 arabinose 5-phosphate isomerase KdsD -
  LMA00_RS14195 (LMA00_14195) - 3036001..3036537 (+) 537 WP_105787193.1 KdsC family phosphatase -
  LMA00_RS14200 (LMA00_14200) lptC 3036541..3037155 (+) 615 WP_006752623.1 LPS export ABC transporter periplasmic protein LptC -
  LMA00_RS14205 (LMA00_14205) lptA 3037176..3037841 (+) 666 WP_006752624.1 lipopolysaccharide transport periplasmic protein LptA -
  LMA00_RS14210 (LMA00_14210) lptB 3037838..3038614 (+) 777 WP_006752625.1 LPS export ABC transporter ATP-binding protein -
  LMA00_RS14215 (LMA00_14215) rpoN/rpoN1 3038785..3040290 (+) 1506 WP_006752626.1 RNA polymerase factor sigma-54 Machinery gene
  LMA00_RS14220 (LMA00_14220) hpf 3040448..3040807 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LMA00_RS14225 (LMA00_14225) - 3040978..3041517 (+) 540 WP_006752627.1 PTS sugar transporter subunit IIA -
  LMA00_RS14230 (LMA00_14230) hprK 3041647..3042615 (+) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  LMA00_RS14235 (LMA00_14235) rapZ 3042691..3043599 (+) 909 WP_041489859.1 RNase adapter RapZ -
  LMA00_RS14240 (LMA00_14240) - 3043626..3044261 (+) 636 WP_006752630.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54757.89 Da        Isoelectric Point: 4.8795

>NTDB_id=624247 LMA00_RS14215 WP_006752626.1 3038785..3040290(+) (rpoN/rpoN1) [Burkholderia ambifaria strain B21-004]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDEWITSPLRVAADGSLIAQGPSGQNIEPGP
ANGSSQSSERAERDEPAGADEYDGYGSGDAGDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASLDEVLTDLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=624247 LMA00_RS14215 WP_006752626.1 3038785..3040290(+) (rpoN/rpoN1) [Burkholderia ambifaria strain B21-004]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
ACTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAGAACGAAGACGAATGGA
TCACGAGTCCGCTGCGTGTCGCGGCGGACGGATCGCTGATCGCGCAGGGGCCTTCCGGACAGAACATCGAGCCAGGCCCC
GCGAACGGCAGCAGCCAGTCGAGCGAGCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
GGGCGACGCCGGCGACGGCCCGCAGTGGAATCTGGACGAGTACGGCCGCTCGAGCGGCGCGTCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCACTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTACT
CACCGACCTGCCGCCGGAACTGGAAGTCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGTTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGGCTCGATCCGTCCCCCACCCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCACCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AACGTCAAAAGAATTTCTTCGCACATGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGTACGCTGGAATTCAAGTACTT
CTTCGGGTCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691