Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LMA02_RS14370 Genome accession   NZ_CP086301
Coordinates   3084160..3085665 (+) Length   501 a.a.
NCBI ID   WP_228905732.1    Uniprot ID   -
Organism   Burkholderia sp. B21-005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3079160..3090665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMA02_RS14345 (LMA02_14355) kdsD 3080393..3081376 (+) 984 WP_166723797.1 arabinose 5-phosphate isomerase KdsD -
  LMA02_RS14350 (LMA02_14360) - 3081376..3081912 (+) 537 WP_228905728.1 KdsC family phosphatase -
  LMA02_RS14355 (LMA02_14365) lptC 3081916..3082530 (+) 615 WP_228905730.1 LPS export ABC transporter periplasmic protein LptC -
  LMA02_RS14360 (LMA02_14370) lptA 3082551..3083216 (+) 666 WP_228905731.1 lipopolysaccharide transport periplasmic protein LptA -
  LMA02_RS14365 (LMA02_14375) lptB 3083213..3083989 (+) 777 WP_166723800.1 LPS export ABC transporter ATP-binding protein -
  LMA02_RS14370 (LMA02_14380) rpoN/rpoN1 3084160..3085665 (+) 1506 WP_228905732.1 RNA polymerase factor sigma-54 Machinery gene
  LMA02_RS14375 (LMA02_14385) hpf 3085824..3086183 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LMA02_RS14380 (LMA02_14390) - 3086353..3086892 (+) 540 WP_006752627.1 PTS sugar transporter subunit IIA -
  LMA02_RS14385 (LMA02_14395) hprK 3087022..3087990 (+) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  LMA02_RS14390 (LMA02_14400) rapZ 3088066..3088974 (+) 909 WP_228905733.1 RNase adapter RapZ -
  LMA02_RS14395 (LMA02_14405) - 3089001..3089636 (+) 636 WP_228905734.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54816.91 Da        Isoelectric Point: 4.8767

>NTDB_id=624232 LMA02_RS14370 WP_228905732.1 3084160..3085665(+) (rpoN/rpoN1) [Burkholderia sp. B21-005]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDEWISSPLRVASDGSLIAQAPSGQNIEPGP
ANSSSQSSDRAERDEPAGADEYDGYGSGDAGDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASLDEVLNDLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=624232 LMA02_RS14370 WP_228905732.1 3084160..3085665(+) (rpoN/rpoN1) [Burkholderia sp. B21-005]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
ACTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCACAGAATCCGCTGCTCGAGAACGAAGACGAATGGA
TCTCGAGTCCGCTGCGTGTCGCGTCGGACGGATCGCTGATTGCGCAGGCGCCTTCCGGACAGAACATCGAGCCGGGCCCC
GCGAACAGCAGTAGCCAGTCGAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
GGGCGACGCCGGCGACGGCCCGCAGTGGAATCTCGACGAGTACGGCCGCTCGAGCGGCGCCTCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCTGGCCCG
CGCGATCGCGCACTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCCTCGCTCGACGAAGTGCT
CAACGACCTGCCGCCGGAACTGGAAATCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGTTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCCTCCGAGTGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCCACCCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCACCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCACACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATTCTGCGTGTCGCGCAGGCCATTGTCG
AACGTCAAAAGAATTTCTTCGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGTACGCTGGAATTCAAGTACTT
CTTCGGGTCACACGTTTCGACCGACACCGGGGGCGCCGCATCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCAGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.317

100

0.689