Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LL998_RS14275 Genome accession   NZ_CP086297
Coordinates   3065671..3067176 (+) Length   501 a.a.
NCBI ID   WP_175670613.1    Uniprot ID   -
Organism   Burkholderia ambifaria strain B21-006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3060671..3072176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL998_RS14250 (LL998_14260) kdsD 3061904..3062887 (+) 984 WP_126228526.1 arabinose 5-phosphate isomerase KdsD -
  LL998_RS14255 (LL998_14265) - 3062887..3063423 (+) 537 WP_011657961.1 KdsC family phosphatase -
  LL998_RS14260 (LL998_14270) lptC 3063427..3064041 (+) 615 WP_229079644.1 LPS export ABC transporter periplasmic protein LptC -
  LL998_RS14265 (LL998_14275) lptA 3064062..3064727 (+) 666 WP_011657963.1 lipopolysaccharide transport periplasmic protein LptA -
  LL998_RS14270 (LL998_14280) lptB 3064724..3065500 (+) 777 WP_011657964.1 LPS export ABC transporter ATP-binding protein -
  LL998_RS14275 (LL998_14285) rpoN/rpoN1 3065671..3067176 (+) 1506 WP_175670613.1 RNA polymerase factor sigma-54 Machinery gene
  LL998_RS14280 (LL998_14290) hpf 3067334..3067693 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LL998_RS14285 (LL998_14295) - 3067863..3068402 (+) 540 WP_011657966.1 PTS sugar transporter subunit IIA -
  LL998_RS14290 (LL998_14300) hprK 3068532..3069500 (+) 969 WP_011657967.1 HPr(Ser) kinase/phosphatase -
  LL998_RS14295 (LL998_14305) rapZ 3069576..3070484 (+) 909 WP_041489859.1 RNase adapter RapZ -
  LL998_RS14300 (LL998_14310) - 3070511..3071146 (+) 636 WP_006752630.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54758.87 Da        Isoelectric Point: 4.8795

>NTDB_id=624217 LL998_RS14275 WP_175670613.1 3065671..3067176(+) (rpoN/rpoN1) [Burkholderia ambifaria strain B21-006]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDEWITSPLRVASDGSLIAQGPSGQNIEPGP
ANGSSPSSERAERDEPAGADEYDGYGSGDAGDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLSDLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=624217 LL998_RS14275 WP_175670613.1 3065671..3067176(+) (rpoN/rpoN1) [Burkholderia ambifaria strain B21-006]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
ACTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAAAATCCGTTGCTCGAAAACGAAGACGAATGGA
TCACGAGTCCGCTGCGTGTCGCATCGGACGGATCGCTGATCGCACAGGGGCCTTCCGGACAGAACATCGAGCCGGGCCCC
GCGAACGGCAGCAGCCCGTCGAGCGAGCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
GGGCGACGCCGGCGACGGCCCGCAGTGGAATCTGGACGAGTACGGCCGCTCGAGCGGCGCGTCGGACGATGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
CTCCGACCTGCCGCCGGAACTGGAAGTCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCCGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGGCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCACCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCGGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGGCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGGTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AACGTCAAAAGAATTTCTTCGCGCATGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGTACGCTGGAGTTCAAGTACTT
CTTCGGGTCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691