Detailed information NTG0062380

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: FNP71_RS13970
• Genome accession: NZ_AP019714
• Coordinates: 2635813..2636430 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: P39694
• Organism: Bacillus subtilis subsp. subtilis strain NBRC 13719
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2630813..2641430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FNP71_RS13940 (NBRC13719_26330)	rpsT	2631115..2631381 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
FNP71_RS13945 (NBRC13719_26340)	holA	2631396..2632439 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
FNP71_RS13950	-	2632479..2632628 (-)	150	WP_003229985.1	hypothetical protein	-
FNP71_RS13955 (NBRC13719_26350)	yqzM	2632669..2632803 (+)	135	WP_003229983.1	YqzM family protein	-
FNP71_RS13960	comEC	2632843..2635173 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
FNP71_RS13965 (NBRC13719_26380)	comEB	2635177..2635746 (-)	570	WP_003229978.1	ComE operon protein 2	-
FNP71_RS13970 (NBRC13719_26390)	comEA	2635813..2636430 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
FNP71_RS13975 (NBRC13719_26400)	comER	2636514..2637335 (+)	822	WP_004398597.1	late competence protein ComER	-
FNP71_RS13980 (NBRC13719_26410)	yqeM	2637401..2638144 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
FNP71_RS13985 (NBRC13719_26420)	rsfS	2638141..2638497 (-)	357	WP_003229971.1	ribosome silencing factor	-
FNP71_RS13990 (NBRC13719_26430)	yqeK	2638515..2639075 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
FNP71_RS13995 (NBRC13719_26440)	nadD	2639065..2639634 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
FNP71_RS14000 (NBRC13719_26450)	yhbY	2639646..2639936 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
FNP71_RS14005 (NBRC13719_26460)	aroE	2639930..2640772 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=62380 FNP71_RS13970 WP_004398514.1 2635813..2636430(-) (comEA) [Bacillus subtilis subsp. subtilis strain NBRC 13719]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=62380 FNP71_RS13970 WP_004398514.1 2635813..2636430(-) (comEA) [Bacillus subtilis subsp. subtilis strain NBRC 13719]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

3D structure

• PDB: 8DFK

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39