Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5T36_RS18550 Genome accession   NZ_CP103623
Coordinates   3845244..3845684 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 3985     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3840244..3850684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T36_RS18530 (M5T36_18530) aroP 3841242..3842615 (+) 1374 WP_001331235.1 aromatic amino acid transporter AroP -
  M5T36_RS18535 (M5T36_18535) ampE 3842658..3843512 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  M5T36_RS18540 (M5T36_18540) ampD 3843509..3844060 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5T36_RS18545 (M5T36_18545) nadC 3844148..3845041 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5T36_RS18550 (M5T36_18550) pilA 3845244..3845684 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  M5T36_RS18555 (M5T36_18555) pilB 3845694..3847079 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  M5T36_RS18560 (M5T36_18560) hofC 3847069..3848271 (+) 1203 WP_000157234.1 protein transport protein HofC -
  M5T36_RS18565 (M5T36_18565) guaC 3848306..3849349 (-) 1044 WP_001217338.1 GMP reductase -
  M5T36_RS18570 (M5T36_18570) - 3849505..3849549 (-) 45 WP_120795372.1 protein YacM -
  M5T36_RS18575 (M5T36_18575) coaE 3849574..3850194 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=623738 M5T36_RS18550 WP_000360895.1 3845244..3845684(+) (pilA) [Escherichia coli strain 3985]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=623738 M5T36_RS18550 WP_000360895.1 3845244..3845684(+) (pilA) [Escherichia coli strain 3985]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363