Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LJY09_RS22225 Genome accession   NZ_CP086208
Coordinates   4294012..4295064 (-) Length   350 a.a.
NCBI ID   WP_028411611.1    Uniprot ID   -
Organism   Priestia sp. J2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4289012..4300064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJY09_RS22185 - 4290028..4290852 (+) 825 WP_014458232.1 YqhG family protein -
  LJY09_RS22190 - 4290876..4291061 (-) 186 WP_013059170.1 YqzE family protein -
  LJY09_RS22195 comGG 4291105..4291479 (-) 375 WP_013059171.1 competence type IV pilus minor pilin ComGG -
  LJY09_RS22200 comGF 4291469..4291906 (-) 438 WP_014458231.1 competence type IV pilus minor pilin ComGF -
  LJY09_RS22205 - 4291903..4292241 (-) 339 WP_028411610.1 type II secretion system protein -
  LJY09_RS22210 comGD 4292225..4292707 (-) 483 WP_226554895.1 competence type IV pilus minor pilin ComGD -
  LJY09_RS22215 comGC 4292649..4292960 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  LJY09_RS22220 comGB 4292975..4294021 (-) 1047 WP_014458228.1 competence type IV pilus assembly protein ComGB -
  LJY09_RS22225 comGA 4294012..4295064 (-) 1053 WP_028411611.1 competence type IV pilus ATPase ComGA Machinery gene
  LJY09_RS22230 - 4295334..4295717 (-) 384 WP_028411612.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LJY09_RS22235 - 4295979..4296215 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  LJY09_RS22240 - 4296253..4296885 (-) 633 WP_028411613.1 MBL fold metallo-hydrolase -
  LJY09_RS22245 - 4297095..4297271 (+) 177 WP_028411614.1 DUF2759 domain-containing protein -
  LJY09_RS22250 - 4297307..4298209 (-) 903 WP_028411615.1 M14 family metallocarboxypeptidase -
  LJY09_RS22255 - 4298321..4299289 (-) 969 WP_098112947.1 ROK family glucokinase -
  LJY09_RS22260 - 4299292..4299498 (-) 207 WP_028411617.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39596.97 Da        Isoelectric Point: 9.0586

>NTDB_id=623729 LJY09_RS22225 WP_028411611.1 4294012..4295064(-) (comGA) [Priestia sp. J2]
MTEIEQFATYVIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSVDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=623729 LJY09_RS22225 WP_028411611.1 4294012..4295064(-) (comGA) [Priestia sp. J2]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATGTGAGTGTCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGGGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCGTTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACCTCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTGCA
GGTAAATGAACGCGCAGGGATTACGTATACGACTGGTTTAAAAGCTATATTGCGCCATGATCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACCGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGCGTTAGCTTTCAAGAAATGGAGCAGACTCTTGTAGC
AGTAGCTGCACAGCGTCTTGTAGAAATTCGCTGTCCGCTGTGTTCGGGAAAATGCCACCCTGCTTGTAAAAAAATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586