Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5S83_RS22395 Genome accession   NZ_CP103609
Coordinates   4628698..4630191 (-) Length   497 a.a.
NCBI ID   WP_001040630.1    Uniprot ID   A7ZT00
Organism   Escherichia coli strain 4314     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4623698..4635191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S83_RS22370 (M5S83_22370) panM 4623921..4624304 (+) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
  M5S83_RS22375 (M5S83_22375) livJ 4624503..4625606 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  M5S83_RS22380 (M5S83_22380) rpoH 4625877..4626731 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  M5S83_RS22385 (M5S83_22385) ftsX 4626976..4628034 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  M5S83_RS22390 (M5S83_22390) ftsE 4628027..4628695 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  M5S83_RS22395 (M5S83_22395) pilA 4628698..4630191 (-) 1494 WP_001040630.1 signal recognition particle-docking protein FtsY Machinery gene
  M5S83_RS22400 (M5S83_22400) rsmD 4630341..4630937 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  M5S83_RS22405 (M5S83_22405) yhhL 4630927..4631196 (+) 270 WP_001295207.1 DUF1145 family protein -
  M5S83_RS22410 (M5S83_22410) yhhM 4631199..4631558 (-) 360 WP_000042901.1 DUF2500 domain-containing protein -
  M5S83_RS22415 (M5S83_22415) yhhN 4631699..4632325 (+) 627 WP_000964718.1 lysoplasmalogenase -
  M5S83_RS22420 (M5S83_22420) zntA 4632399..4634597 (+) 2199 WP_023155844.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  M5S83_RS22425 (M5S83_22425) tusA 4634699..4634944 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54530.33 Da        Isoelectric Point: 4.1560

>NTDB_id=623676 M5S83_RS22395 WP_001040630.1 4628698..4630191(-) (pilA) [Escherichia coli strain 4314]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETAEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=623676 M5S83_RS22395 WP_001040630.1 4628698..4630191(-) (pilA) [Escherichia coli strain 4314]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAACCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGGCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGTAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGATGTG
GGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGT
TCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGTAA
CAATATCCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCGGTTATCTTCGACGCCATTCAGGCTGCGAAGGCGC
GTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.654

81.489

0.388