Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5T00_RS20200 Genome accession   NZ_CP103589
Coordinates   4156362..4156802 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 4750     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4151362..4161802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T00_RS20180 (M5T00_20180) aroP 4152362..4153732 (+) 1371 WP_001540704.1 aromatic amino acid transporter AroP -
  M5T00_RS20185 (M5T00_20185) ampE 4153775..4154629 (-) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  M5T00_RS20190 (M5T00_20190) ampD 4154626..4155177 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5T00_RS20195 (M5T00_20195) nadC 4155265..4156158 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5T00_RS20200 (M5T00_20200) pilA 4156362..4156802 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  M5T00_RS20205 (M5T00_20205) pilB 4156812..4158197 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  M5T00_RS20210 (M5T00_20210) hofC 4158187..4159389 (+) 1203 WP_001540700.1 protein transport protein HofC -
  M5T00_RS20215 (M5T00_20215) guaC 4159424..4160467 (-) 1044 WP_001217338.1 GMP reductase -
  M5T00_RS20220 (M5T00_20220) - 4160623..4160667 (-) 45 WP_120795372.1 protein YacM -
  M5T00_RS20225 (M5T00_20225) coaE 4160692..4161312 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=623405 M5T00_RS20200 WP_000360914.1 4156362..4156802(+) (pilA) [Escherichia coli strain 4750]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=623405 M5T00_RS20200 WP_000360914.1 4156362..4156802(+) (pilA) [Escherichia coli strain 4750]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363