Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5S79_RS18230 Genome accession   NZ_CP103570
Coordinates   3790412..3790852 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 3045     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3785412..3795852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S79_RS18210 (M5S79_18210) aroP 3786410..3787783 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M5S79_RS18215 (M5S79_18215) ampE 3787826..3788680 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  M5S79_RS18220 (M5S79_18220) ampD 3788677..3789228 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5S79_RS18225 (M5S79_18225) nadC 3789316..3790209 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5S79_RS18230 (M5S79_18230) pilA 3790412..3790852 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  M5S79_RS18235 (M5S79_18235) pilB 3790862..3792247 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  M5S79_RS18240 (M5S79_18240) hofC 3792237..3793439 (+) 1203 WP_000157266.1 protein transport protein HofC -
  M5S79_RS18245 (M5S79_18245) guaC 3793474..3794517 (-) 1044 WP_001217338.1 GMP reductase -
  M5S79_RS18250 (M5S79_18250) - 3794673..3794717 (-) 45 WP_120795372.1 protein YacM -
  M5S79_RS18255 (M5S79_18255) coaE 3794742..3795362 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=623322 M5S79_RS18230 WP_000360904.1 3790412..3790852(+) (pilA) [Escherichia coli strain 3045]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=623322 M5S79_RS18230 WP_000360904.1 3790412..3790852(+) (pilA) [Escherichia coli strain 3045]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363