Detailed information NTG0623269

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: M5S79_RS01315
• Genome accession: NZ_CP103570
• Coordinates: 281893..283386 (+)
• Length: 497 a.a.
• NCBI ID: WP_001040654.1
• Uniprot ID: P10121
• Organism: Escherichia coli strain 3045
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 276893..288386

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S79_RS01285 (M5S79_01285)	tusA	277140..277385 (+)	246	WP_000130621.1	sulfurtransferase TusA	-
M5S79_RS01290 (M5S79_01290)	zntA	277487..279685 (-)	2199	WP_046464157.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
M5S79_RS01295 (M5S79_01295)	yhhN	279759..280385 (-)	627	WP_000964718.1	lysoplasmalogenase	-
M5S79_RS01300 (M5S79_01300)	yhhM	280526..280885 (+)	360	WP_000042900.1	DUF2500 domain-containing protein	-
M5S79_RS01305 (M5S79_01305)	yhhL	280888..281157 (-)	270	WP_001295207.1	DUF1145 family protein	-
M5S79_RS01310 (M5S79_01310)	rsmD	281147..281743 (-)	597	WP_000743193.1	16S rRNA (guanine(966)-N(2))-methyltransferase	-
M5S79_RS01315 (M5S79_01315)	pilA	281893..283386 (+)	1494	WP_001040654.1	signal recognition particle-docking protein FtsY	Machinery gene
M5S79_RS01320 (M5S79_01320)	ftsE	283389..284057 (+)	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
M5S79_RS01325 (M5S79_01325)	ftsX	284050..285108 (+)	1059	WP_001042003.1	permease-like cell division protein FtsX	-
M5S79_RS01330 (M5S79_01330)	rpoH	285353..286207 (+)	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
M5S79_RS01335 (M5S79_01335)	livJ	286478..287581 (+)	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
M5S79_RS01340 (M5S79_01340)	panM	287780..288163 (-)	384	WP_000778785.1	aspartate 1-decarboxylase autocleavage activator PanM	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 54513.35 Da
• Isoelectric Point: 4.1560

>NTDB_id=623269 M5S79_RS01315 WP_001040654.1 281893..283386(+) (pilA) [Escherichia coli strain 3045]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide

• Length: 1494 bp

>NTDB_id=623269 M5S79_RS01315 WP_001040654.1 281893..283386(+) (pilA) [Escherichia coli strain 3045]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (210-275)

• SRP54-type protein, GTPase domain (SRP54) (293-493)

3D structure

• PDB: 1FTS
• PDB: 2QY9
• PDB: 2XXA
• PDB: 2YHS
• PDB: 3ZN8
• PDB: 4C7O
• PDB: 5GAD
• PDB: 5NCO
• PDB: 5NIY
• PDB: 6CQP
• PDB: 6CS8
• PDB: 6CVD
• PDB: 6DLX
• PDB: 6FPK
• PDB: 6FPR
• PDB: 6FQD
• PDB: 6N5I
• PDB: 6N5J
• PDB: 6N6N
• PDB: 6N9B
• PDB: 6NC1
• PDB: 6NC4
• PDB: 7O9H

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	48.698	77.264	0.376