Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5R24_RS20475 Genome accession   NZ_CP103562
Coordinates   4137484..4137924 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli O25b:H4-ST131 strain 751     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4132484..4142924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5R24_RS20455 (M5R24_20455) aroP 4133484..4134854 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  M5R24_RS20460 (M5R24_20460) ampE 4134897..4135751 (-) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  M5R24_RS20465 (M5R24_20465) ampD 4135748..4136299 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5R24_RS20470 (M5R24_20470) nadC 4136387..4137280 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5R24_RS20475 (M5R24_20475) pilA 4137484..4137924 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  M5R24_RS20480 (M5R24_20480) gspE 4137934..4139319 (+) 1386 Protein_4042 type II secretion system protein GspE -
  M5R24_RS20485 (M5R24_20485) hofC 4139309..4140511 (+) 1203 WP_000157289.1 protein transport protein HofC -
  M5R24_RS20490 (M5R24_20490) guaC 4140546..4141589 (-) 1044 WP_001217320.1 GMP reductase -
  M5R24_RS20495 (M5R24_20495) - 4141745..4141789 (-) 45 WP_120795372.1 protein YacM -
  M5R24_RS20500 (M5R24_20500) coaE 4141814..4142434 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=623238 M5R24_RS20475 WP_000360914.1 4137484..4137924(+) (pilA) [Escherichia coli O25b:H4-ST131 strain 751]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=623238 M5R24_RS20475 WP_000360914.1 4137484..4137924(+) (pilA) [Escherichia coli O25b:H4-ST131 strain 751]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363