Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KW2_RS10540 Genome accession   NC_022369
Coordinates   2199912..2201273 (-) Length   453 a.a.
NCBI ID   WP_011677138.1    Uniprot ID   A0A165G707
Organism   Lactococcus cremoris subsp. cremoris KW2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2194912..2206273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS10505 (kw2_2115) - 2195435..2195929 (+) 495 WP_041168746.1 GNAT family N-acetyltransferase -
  KW2_RS10510 (kw2_2116) - 2196020..2196343 (-) 324 WP_021037923.1 DUF1048 domain-containing protein -
  KW2_RS10515 (kw2_2117) - 2196340..2196678 (-) 339 WP_021037924.1 DUF1048 domain-containing protein -
  KW2_RS10520 (kw2_2118) - 2196671..2197027 (-) 357 WP_021037925.1 PadR family transcriptional regulator -
  KW2_RS10525 (kw2_2119) - 2197149..2197778 (-) 630 WP_021037926.1 hypothetical protein -
  KW2_RS10530 (kw2_2120) - 2197837..2198922 (-) 1086 WP_021037927.1 PIN/TRAM domain-containing protein -
  KW2_RS10535 (kw2_2121) - 2199189..2199866 (+) 678 WP_021037928.1 YoaK family protein -
  KW2_RS10540 (kw2_2122) radA 2199912..2201273 (-) 1362 WP_011677138.1 DNA repair protein RadA Machinery gene
  KW2_RS10545 (kw2_2123) - 2201443..2202456 (-) 1014 WP_021037929.1 glycosyltransferase family 2 protein -
  KW2_RS10550 (kw2_2124) - 2202438..2202983 (-) 546 WP_021037930.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  KW2_RS10555 (kw2_2125) - 2202980..2203555 (-) 576 WP_021037931.1 SIS domain-containing protein -
  KW2_RS10560 (kw2_2126) - 2203617..2204591 (-) 975 WP_021037932.1 GHMP kinase -
  KW2_RS10565 (kw2_2127) - 2204621..2206144 (-) 1524 WP_021037933.1 lipopolysaccharide biosynthesis protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49897.33 Da        Isoelectric Point: 5.2965

>NTDB_id=62281 KW2_RS10540 WP_011677138.1 2199912..2201273(-) (radA) [Lactococcus cremoris subsp. cremoris KW2]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMSGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIKVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=62281 KW2_RS10540 WP_011677138.1 2199912..2201273(-) (radA) [Lactococcus cremoris subsp. cremoris KW2]
ATGGCGAAGAAAAAATCATCTTTTGTTTGTCAAAATTGTGGTTATAAGTCTGCAAAGTATCTTGGGCGATGCCCAAATTG
TGGAGAGTGGTCCTCTTTTGTTGAAGAAGTTGAAGTACAGGAAGTCAAAAATCAGCGAGTGTCGATGTCTGGTGAACGTT
CAAAACCAATGAAATTAGATGAAGTTGAGCTTTTTGATACACCACGTATTGAAACAGATCTTGACGAATTTAATCGTGTT
TTAGGTGGTGGTGTTGTTCCTGGTTCACTCGTTTTGATTGGTGGAGACCCAGGAATAGGAAAATCTACGTTGCTTTTGCA
AGTATCAACTCAGCTTGCATCAAGAGGACGGGTTCTCTATGTGAGTGGTGAAGAATCTGCTCAACAAATTAAGCTTAGAG
CGGAACGTTTGGGCGATATTGATCGAGATTTTTATCTTTACGCCGAGACTAACATGCAGTCAATTCGTGCTGAAGTTGAA
CGTTTGAAGCCAAATTTTTTGATTATTGATTCGATCCAAACGATTATGACACCTGAAATTCAATCAACTCAAGGCTCTGT
GAGTCAAGTTCGAGAAGTGACAGGTGAACTCATGCAAATTGCCAAAACGAATGATATTGCTACTTTTATCGTTGGTCATG
TGACGAAAGAAGGTCAATTGGCAGGACCAAGAATGCTAGAGCACATGGTTGATACCGTTCTTTATTTTGAAGGAGAAAGA
AATAATACTTTTAGAATTCTTCGAGCAGTTAAAAACCGGTTTGGGTCAACTAATGAAATTGGTATATTTGAAATGCAAGG
GCATGGTTTAGTTGAAGTAACTAATCCTTCTGAGGTTTTCTTGGAAGAGCGATTAGAGGGTTCAACTGGTTCTGCTATTG
TTTGTGCACTCGAAGGGACACGTCCGATACTAGTTGAAATACAGGCTTTAACTACACCAACAATGTTTGGAAATGCTAAG
AGAACAACTTCTGGTTTAGATTTTAATAGAGTGAGCCTGATTATGGCAGTACTTGAAAAACGGACAGGCTTATTGATGCA
AAATCAAGATGCTTACCTTAAATCAGCTGGGGGAGTAAAATTAGATGAGCCAGCCATTGACCTTGCGGTTGCTGTCGCTG
TAGCCTCAAGTTATAAAGAACTTCCGACGGATGCGCGTGAATGTTTTATTGGTGAAATTGGCTTAACAGGAGAGATTCGA
CGTGTGACGCGAATTGAACAAAGACTGAATGAAGCTGCTAAATTAGGATTCAAAAAAGTCTATGCTCCTAAAAATTCAAT
TGTGGGAATTGATATTCCTGAACAAATAAAAGTAATTGGGGTAACAACCCTAACTGAATGTCTAAAATTAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A165G707

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.707

100

0.623

Multiple sequence alignment