Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   M5S63_RS24150 Genome accession   NZ_CP103521
Coordinates   4990427..4992256 (-) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli strain 2779 - blood draw same patient as MB9267     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4985427..4997256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S63_RS24125 (M5S63_24125) bioP 4986145..4987044 (-) 900 WP_001551984.1 biotin transporter -
  M5S63_RS24130 (M5S63_24130) yigL 4987120..4987920 (-) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  M5S63_RS24135 (M5S63_24135) pldB 4987928..4988950 (-) 1023 WP_020233319.1 lysophospholipase L2 -
  M5S63_RS24140 (M5S63_24140) rhtB 4989061..4989681 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  M5S63_RS24145 (M5S63_24145) rhtC 4989743..4990363 (-) 621 WP_001521771.1 threonine export protein RhtC -
  M5S63_RS24150 (M5S63_24150) recQ 4990427..4992256 (-) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  M5S63_RS24155 (M5S63_24155) pldA 4992389..4993258 (-) 870 WP_001521770.1 phospholipase A -
  M5S63_RS24160 (M5S63_24160) yigI 4993423..4993890 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  M5S63_RS24165 (M5S63_24165) rarD 4993942..4994832 (+) 891 WP_000339104.1 EamA family transporter RarD -
  M5S63_RS24170 (M5S63_24170) - 4995143..4995991 (+) 849 WP_021516929.1 YPO3983 family protein -
  M5S63_RS24175 (M5S63_24175) - 4995988..4996479 (+) 492 WP_000837562.1 DUF943 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=622677 M5S63_RS24150 WP_000035581.1 4990427..4992256(-) (recQ) [Escherichia coli strain 2779 - blood draw same patient as MB9267]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=622677 M5S63_RS24150 WP_000035581.1 4990427..4992256(-) (recQ) [Escherichia coli strain 2779 - blood draw same patient as MB9267]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
TATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGTCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGTTTTCCGACGCTGCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGTTATGTACAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGTAATAGTCGTGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAACATTGAATCCTAT
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCGGATATGGCGTG
GCTGCGTCGTTGTCTGGAAGAGAAACCGCAGGGGCAGTTACAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTGCTGCTGAACTACTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
TTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCACGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCCTTCGGCGGCAACTACGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCACATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391