Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5S96_RS18685 Genome accession   NZ_CP103515
Coordinates   3817210..3817650 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 5444     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3812210..3822650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S96_RS18665 (M5S96_18665) aroP 3813208..3814581 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M5S96_RS18670 (M5S96_18670) ampE 3814624..3815478 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  M5S96_RS18675 (M5S96_18675) ampD 3815475..3816026 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5S96_RS18680 (M5S96_18680) nadC 3816114..3817007 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5S96_RS18685 (M5S96_18685) pilA 3817210..3817650 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  M5S96_RS18690 (M5S96_18690) pilB 3817660..3819045 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  M5S96_RS18695 (M5S96_18695) hofC 3819035..3820237 (+) 1203 WP_000157266.1 protein transport protein HofC -
  M5S96_RS18700 (M5S96_18700) guaC 3820272..3821315 (-) 1044 WP_001217338.1 GMP reductase -
  M5S96_RS18705 (M5S96_18705) - 3821471..3821515 (-) 45 WP_120795372.1 protein YacM -
  M5S96_RS18710 (M5S96_18710) coaE 3821540..3822160 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=622575 M5S96_RS18685 WP_000360904.1 3817210..3817650(+) (pilA) [Escherichia coli strain 5444]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=622575 M5S96_RS18685 WP_000360904.1 3817210..3817650(+) (pilA) [Escherichia coli strain 5444]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363