Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   M5T46_RS02395 Genome accession   NZ_CP103479
Coordinates   518301..518768 (-) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain 4621     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 513301..523768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T46_RS02370 (M5T46_02370) rpsG 513442..513912 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  M5T46_RS02375 (M5T46_02375) fusA 514009..516123 (+) 2115 WP_000124700.1 elongation factor G -
  M5T46_RS02380 (M5T46_02380) tuf 516194..517378 (+) 1185 WP_000031783.1 elongation factor Tu -
  M5T46_RS02385 (M5T46_02385) bfd 517561..517755 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  M5T46_RS02390 (M5T46_02390) bfr 517828..518304 (+) 477 WP_000675515.1 bacterioferritin -
  M5T46_RS02395 (M5T46_02395) pilD 518301..518768 (-) 468 WP_000340159.1 A24 family peptidase Machinery gene
  M5T46_RS02400 (M5T46_02400) rpsJ 519147..519458 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  M5T46_RS02405 (M5T46_02405) rplC 519491..520120 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  M5T46_RS02410 (M5T46_02410) rplD 520131..520736 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  M5T46_RS02415 (M5T46_02415) rplW 520733..521035 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  M5T46_RS02420 (M5T46_02420) rplB 521053..521874 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  M5T46_RS02425 (M5T46_02425) rpsS 521891..522169 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  M5T46_RS02430 (M5T46_02430) rplV 522184..522516 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  M5T46_RS02435 (M5T46_02435) rpsC 522534..523235 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  M5T46_RS02440 (M5T46_02440) rplP 523248..523658 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=622360 M5T46_RS02395 WP_000340159.1 518301..518768(-) (pilD) [Escherichia coli strain 4621]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=622360 M5T46_RS02395 WP_000340159.1 518301..518768(-) (pilD) [Escherichia coli strain 4621]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368