Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   M5S50_RS24405 Genome accession   NZ_CP103468
Coordinates   5036257..5038086 (-) Length   609 a.a.
NCBI ID   WP_024179510.1    Uniprot ID   -
Organism   Escherichia coli strain 4973     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5031257..5043086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S50_RS24380 (M5S50_24375) yigL 5031956..5032756 (-) 801 WP_000285349.1 sugar/pyridoxal phosphate phosphatase YigL -
  M5S50_RS24385 (M5S50_24380) pldB 5032764..5033786 (-) 1023 WP_022646339.1 lysophospholipase L2 -
  M5S50_RS24390 (M5S50_24385) rhtB 5033897..5034517 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  M5S50_RS24395 (M5S50_24390) - 5034676..5035536 (+) 861 WP_000133649.1 Rpn family recombination-promoting nuclease/putative transposase -
  M5S50_RS24400 (M5S50_24395) rhtC 5035573..5036193 (-) 621 WP_000928837.1 threonine export protein RhtC -
  M5S50_RS24405 (M5S50_24400) recQ 5036257..5038086 (-) 1830 WP_024179510.1 ATP-dependent DNA helicase RecQ Machinery gene
  M5S50_RS24410 (M5S50_24405) pldA 5038211..5039080 (-) 870 WP_001259693.1 phospholipase A -
  M5S50_RS24415 (M5S50_24410) yigI 5039245..5039712 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  M5S50_RS24420 (M5S50_24415) rarD 5039764..5040654 (+) 891 WP_000339104.1 EamA family transporter RarD -
  M5S50_RS24425 (M5S50_24420) yigG 5040749..5041129 (+) 381 WP_021521448.1 protein YigG -
  M5S50_RS24430 (M5S50_24425) yigF 5041143..5041523 (+) 381 Protein_4795 DUF2628 domain-containing protein -
  M5S50_RS24435 (M5S50_24430) - 5041566..5041712 (-) 147 Protein_4796 CorA family divalent cation transporter -
  M5S50_RS24440 (M5S50_24435) - 5041899..5042747 (+) 849 WP_022646337.1 YPO3983 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68391.56 Da        Isoelectric Point: 7.2543

>NTDB_id=622343 M5S50_RS24405 WP_024179510.1 5036257..5038086(-) (recQ) [Escherichia coli strain 4973]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLDVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNIRVDVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=622343 M5S50_RS24405 WP_024179510.1 5036257..5038086(-) (recQ) [Escherichia coli strain 4973]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGGGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACCGTACTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGACGT
GATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTGTATATCGCGCCGGAACGCCTGATGCTGGATAACTTCCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGGTTCCCGACGCTCCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGGCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGATCGCCCGAACATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGCAACAGCCGGGCGAAAGTCGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATATTCGCGTCGACGTGCAGGAAAAATTTCAGCGCGATGACCTGCAAATTGTGGTGGCGACCG
TGGCGTTTGGCATGGGCATCAACAAGCCTAACGTCCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTACGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAATTGCAGGATATCGAGCGCCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTGCTGCTGAACTACTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTATTTAACGAC
GCAACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

39.865

97.209

0.388