Detailed information NTG0622340

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL047_RS09395
• Genome accession: NZ_CP086072
• Coordinates: 1857992..1858639 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain EIP33
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1852992..1863639

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL047_RS09370 (LL047_09345)	atpF	1853062..1853568 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LL047_RS09375 (LL047_09350)	atpB	1853583..1854296 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LL047_RS09380 (LL047_09355)	-	1854341..1854556 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL047_RS09385 (LL047_09360)	-	1854740..1855516 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LL047_RS09390 (LL047_09365)	comEC	1855801..1858011 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL047_RS09395 (LL047_09370)	comEA	1857992..1858639 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LL047_RS09400 (LL047_09375)	-	1858699..1860060 (-)	1362	WP_021214597.1	ABC transporter permease	-
LL047_RS09405 (LL047_09380)	-	1860057..1860989 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LL047_RS09410 (LL047_09385)	-	1861094..1861492 (-)	399	WP_347688968.1	hypothetical protein	-
LL047_RS09415 (LL047_09390)	-	1861596..1862159 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LL047_RS09420 (LL047_09395)	-	1862332..1863510 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=622340 LL047_RS09395 WP_010906131.1 1857992..1858639(-) (comEA) [Lactococcus lactis subsp. lactis strain EIP33]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=622340 LL047_RS09395 WP_010906131.1 1857992..1858639(-) (comEA) [Lactococcus lactis subsp. lactis strain EIP33]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386