Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LK685_RS08050 Genome accession   NZ_CP086061
Coordinates   1552180..1552590 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. BC1-43     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1547180..1557590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK685_RS08010 (LK685_08015) arsC 1547475..1547894 (+) 420 WP_029318100.1 arsenate reductase (thioredoxin) -
  LK685_RS08015 (LK685_08020) - 1548105..1548338 (-) 234 Protein_1571 helix-turn-helix domain-containing protein -
  LK685_RS08020 (LK685_08025) - 1548677..1549672 (-) 996 WP_029318099.1 acryloyl-CoA reductase -
  LK685_RS08025 (LK685_08030) - 1549830..1550423 (+) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  LK685_RS08030 (LK685_08035) - 1550856..1551020 (+) 165 WP_080265384.1 hypothetical protein -
  LK685_RS08035 (LK685_08040) - 1550978..1551174 (+) 197 Protein_1575 recombinase family protein -
  LK685_RS08040 (LK685_08045) - 1551140..1551256 (-) 117 WP_122894874.1 hypothetical protein -
  LK685_RS08045 (LK685_08050) sigK 1551256..1551984 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  LK685_RS08050 (LK685_08055) nucA/comI 1552180..1552590 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  LK685_RS08055 (LK685_08060) - 1552623..1553345 (-) 723 WP_010886572.1 hypothetical protein -
  LK685_RS08060 (LK685_08065) gnd 1553597..1554490 (+) 894 WP_043857850.1 decarboxylating 6-phosphogluconate dehydrogenase -
  LK685_RS08065 (LK685_08070) - 1554509..1555135 (-) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  LK685_RS08070 (LK685_08075) cwlH 1555322..1556074 (+) 753 WP_229762700.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  LK685_RS08075 (LK685_08080) - 1556326..1557057 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  LK685_RS08080 (LK685_08085) - 1557363..1557503 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=622167 LK685_RS08050 WP_009967785.1 1552180..1552590(+) (nucA/comI) [Bacillus sp. BC1-43]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=622167 LK685_RS08050 WP_009967785.1 1552180..1552590(+) (nucA/comI) [Bacillus sp. BC1-43]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529