Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LK428_RS09810 Genome accession   NZ_CP085969
Coordinates   2065312..2066751 (-) Length   479 a.a.
NCBI ID   WP_049805778.1    Uniprot ID   -
Organism   Bordetella pertussis strain FDAARGOS_1543     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2060312..2071751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK428_RS09785 (LK428_09785) thpR 2061135..2061701 (+) 567 WP_049805779.1 RNA 2',3'-cyclic phosphodiesterase -
  LK428_RS09790 (LK428_09790) - 2061794..2062744 (+) 951 WP_005013747.1 IS481-like element IS481 family transposase -
  LK428_RS09795 (LK428_09795) - 2062975..2063903 (-) 929 Protein_1928 DMT family transporter -
  LK428_RS09800 (LK428_09800) - 2064011..2064889 (+) 879 WP_010929512.1 LysR substrate-binding domain-containing protein -
  LK428_RS09805 (LK428_09805) - 2064917..2065219 (-) 303 WP_003815905.1 bacterioferritin-associated ferredoxin -
  LK428_RS09810 (LK428_09810) rpoN/rpoN1 2065312..2066751 (-) 1440 WP_049805778.1 RNA polymerase factor sigma-54 Machinery gene
  LK428_RS09815 (LK428_09815) - 2066750..2067397 (+) 648 WP_010929640.1 hypothetical protein -
  LK428_RS09820 (LK428_09820) leuA 2067824..2069524 (+) 1701 WP_010929639.1 2-isopropylmalate synthase -
  LK428_RS09825 (LK428_09825) - 2069615..2070868 (-) 1254 WP_010929638.1 amidase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 52323.55 Da        Isoelectric Point: 4.8131

>NTDB_id=621760 LK428_RS09810 WP_049805778.1 2065312..2066751(-) (rpoN/rpoN1) [Bordetella pertussis strain FDAARGOS_1543]
MQNIVPMTRPALELRPGQHLTLTPQLQQSIRLLQLSSLELEAELSQALAENPLLERDEDPAREEQADPEREASVEADEAP
DTELRLDEMPGSGGVLPDDDSDLPQAARPDTLREHLLGQLALTRAAPRDAALCGLLIDELDENGYLDSPLEEILTWLPAE
VQADLDELRAALALLQSFDPAGIGARDMAECLQLQLRQPDPARLPEAADAEVLACARRICAQHLPLLATGNVARVREAVQ
CDEAVVRAAHALIPRLEPRPGRAWAVPAADYAIPDVIVRRTRQGWQAVLNSAVVPRLHVNAVYAQVLGGQRQGGNAGLQA
QLQQARWMIRNVEQRFDTILRVAQAIVAHQQAFFTQGPAAMRPLILKDIAGELDLHESTISRATTQKYMLTPFGTLELKR
FFGAGVSTDGGDATSATAVQAVIRQMVADEDRAKPLSDSQIMQRLADQGIVIARRTVAKYREALRIAPATLRKAHAAGR

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=621760 LK428_RS09810 WP_049805778.1 2065312..2066751(-) (rpoN/rpoN1) [Bordetella pertussis strain FDAARGOS_1543]
ATGCAAAATATTGTACCTATGACTCGTCCCGCGCTAGAACTTCGTCCTGGTCAACATTTGACGTTGACCCCTCAACTGCA
ACAGTCGATCCGGCTGTTGCAGCTGTCGTCGCTGGAGCTGGAGGCCGAACTGTCGCAGGCGCTGGCCGAGAATCCGTTGC
TGGAACGCGACGAAGATCCGGCGCGCGAGGAGCAGGCCGACCCCGAGCGCGAGGCCTCGGTCGAGGCCGACGAGGCGCCG
GATACCGAACTGCGCCTGGACGAGATGCCCGGTTCGGGCGGCGTTCTTCCCGACGACGACAGCGACCTGCCGCAGGCGGC
GCGCCCGGATACCCTGCGCGAACACCTGCTGGGGCAGCTGGCGCTGACGCGCGCCGCGCCGCGCGATGCGGCGCTGTGCG
GTCTGCTGATCGACGAACTGGACGAGAACGGCTACCTGGATTCGCCCCTGGAAGAGATCCTGACCTGGCTGCCGGCCGAG
GTGCAGGCCGACCTGGACGAGCTGCGCGCGGCGCTGGCATTGCTGCAGTCGTTCGATCCGGCAGGCATCGGCGCGCGCGA
CATGGCCGAATGCCTGCAGCTGCAGTTGCGCCAGCCCGATCCGGCGCGCCTGCCCGAGGCGGCCGATGCCGAGGTGCTGG
CCTGTGCGCGGCGCATCTGCGCGCAGCATCTGCCCTTGCTGGCCACCGGCAACGTGGCCCGCGTGCGCGAGGCCGTGCAA
TGCGACGAGGCGGTCGTGCGCGCCGCGCATGCGCTGATCCCGCGCCTGGAACCGCGGCCGGGCCGCGCCTGGGCGGTGCC
GGCGGCCGATTACGCCATTCCCGACGTGATCGTGCGCCGTACCCGCCAGGGCTGGCAGGCGGTGCTCAACAGCGCCGTCG
TGCCGCGGCTGCATGTCAATGCCGTCTATGCGCAAGTGCTGGGCGGCCAGCGCCAGGGCGGCAACGCCGGCCTGCAGGCG
CAGCTGCAACAGGCGCGCTGGATGATACGCAACGTGGAGCAGCGCTTCGACACCATCCTGCGCGTGGCGCAGGCGATCGT
GGCGCATCAGCAGGCGTTCTTCACCCAGGGGCCGGCGGCCATGCGGCCGCTGATCCTCAAGGACATCGCCGGCGAACTGG
ATCTGCACGAATCGACCATTTCGCGCGCGACGACGCAGAAGTACATGCTCACACCGTTCGGTACGCTGGAGCTCAAGCGC
TTCTTTGGCGCGGGCGTGTCCACCGATGGCGGAGACGCCACCTCGGCCACCGCGGTGCAGGCGGTGATCCGCCAGATGGT
GGCCGACGAGGATCGCGCCAAGCCCTTGTCGGACAGCCAGATCATGCAGCGCCTGGCCGACCAGGGCATCGTCATTGCCC
GCCGCACGGTGGCCAAGTACCGCGAGGCGCTGCGGATTGCGCCAGCGACGCTGCGCAAGGCCCACGCCGCGGGTCGTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-473)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.419

100

0.511