Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HP435_RS01325 Genome accession   NZ_CP085874
Coordinates   230035..231402 (+) Length   455 a.a.
NCBI ID   WP_063231136.1    Uniprot ID   A0A4Y3PV01
Organism   Brevibacillus sp. HD3.3A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 225035..236402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP435_RS01305 (HP435_01315) - 225301..225765 (+) 465 WP_063231140.1 CtsR family transcriptional regulator -
  HP435_RS01310 (HP435_01320) - 225799..226317 (+) 519 WP_063231139.1 UvrB/UvrC motif-containing protein -
  HP435_RS01315 (HP435_01325) - 226338..227408 (+) 1071 WP_122966817.1 protein arginine kinase -
  HP435_RS01320 (HP435_01330) - 227482..229932 (+) 2451 WP_122966816.1 ATP-dependent Clp protease ATP-binding subunit -
  HP435_RS01325 (HP435_01335) radA 230035..231402 (+) 1368 WP_063231136.1 DNA repair protein RadA Machinery gene
  HP435_RS01330 (HP435_01340) disA 231412..232488 (+) 1077 WP_122966815.1 DNA integrity scanning diadenylate cyclase DisA -
  HP435_RS01335 (HP435_01345) pssA 232606..233334 (+) 729 WP_063231134.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  HP435_RS01340 (HP435_01350) - 233428..233823 (-) 396 WP_063231133.1 hypothetical protein -
  HP435_RS01345 (HP435_01355) - 234111..235199 (+) 1089 WP_173599888.1 PIN/TRAM domain-containing protein -
  HP435_RS01350 (HP435_01360) ispD 235238..235924 (+) 687 WP_173599889.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HP435_RS01355 (HP435_01365) ispF 235928..236401 (+) 474 WP_063231130.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49313.84 Da        Isoelectric Point: 7.1367

>NTDB_id=621004 HP435_RS01325 WP_063231136.1 230035..231402(+) (radA) [Brevibacillus sp. HD3.3A]
MSKYKTKYACQECGYESPKWMGKCPGCGSWNTLVEEVTVKAAPRHEGISGGTRQAAIPLTQVASAEEPRMDTTIGELNRV
LGGGLVPGSLILVGGDPGIGKSTLLLQTSFALAHKGAKVLYVSGEESAKQIKLRADRLHMQTPPMFVLAENDLDLIEQHI
NQVDPHVLIIDSIQTVFHPTVQSAAGSVAQVREATAQLMRIAKGKGIGTFIVGHVTKEGSIAGPRMLEHMVDAVLYFEGE
RHNTFRILRAVKNRFGSTNEIGIFEMKDRGLEEVANPSEIFLAERPFGVAGSTVVASMEGTRPVLVELQALVAPTSFVTP
RRMATGVDHQRVAMIMAVLEKRMGMMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEIGLTGEV
RGVSRIEQRVREAHKLGFKRVIIPEKNIRGLDAPDDIQVIGVSNIADALNEVIRR

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=621004 HP435_RS01325 WP_063231136.1 230035..231402(+) (radA) [Brevibacillus sp. HD3.3A]
ATGTCAAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCTAAGTGGATGGGAAAATGCCCTGGCTG
CGGCAGCTGGAACACGTTGGTCGAGGAAGTGACAGTGAAAGCAGCCCCTCGTCATGAAGGAATCAGCGGCGGTACGCGTC
AGGCGGCTATTCCTTTGACGCAGGTCGCCAGTGCAGAAGAACCGCGCATGGACACGACGATCGGAGAGTTGAACCGTGTT
CTCGGCGGCGGGCTGGTACCAGGCTCCCTTATACTGGTCGGCGGCGATCCGGGTATCGGGAAATCTACATTGCTGTTGCA
GACCTCTTTTGCCTTGGCTCATAAAGGGGCCAAAGTGCTGTACGTATCAGGCGAGGAGTCGGCAAAACAAATCAAGCTTC
GCGCTGATCGCCTGCATATGCAAACACCCCCGATGTTTGTGTTGGCAGAAAATGATTTGGATTTGATCGAGCAGCATATT
AATCAAGTAGACCCTCACGTATTGATCATTGACTCGATCCAGACAGTCTTCCATCCCACGGTTCAATCCGCAGCCGGAAG
TGTCGCACAGGTGCGTGAGGCTACCGCTCAACTGATGCGCATCGCCAAAGGCAAAGGGATCGGGACCTTTATTGTCGGCC
ACGTGACCAAAGAAGGTTCGATTGCAGGTCCGCGCATGCTTGAGCATATGGTGGACGCGGTCCTTTATTTTGAAGGCGAG
CGCCACAATACGTTCCGAATCCTGCGTGCTGTCAAAAATCGATTCGGTTCGACAAACGAGATCGGCATTTTCGAGATGAA
AGATCGCGGTCTGGAAGAGGTGGCAAACCCTTCGGAAATTTTTCTGGCCGAACGTCCTTTTGGCGTGGCAGGCTCCACCG
TCGTAGCGAGCATGGAGGGCACTCGTCCCGTCCTGGTCGAGCTTCAGGCACTCGTAGCCCCAACCAGCTTCGTTACGCCG
CGCAGAATGGCTACAGGCGTCGACCATCAGCGTGTGGCGATGATCATGGCTGTTTTGGAAAAGCGCATGGGGATGATGCT
GCAAAATCAGGACGCGTATGTCAATGTCGCAGGGGGCGTTCGTTTGGATGAGCCGGCTGTCGACTTGGCGATCGCGGTCA
GCATTGCCAGCAGCTTCCGCGACCACGCCACGAATCCGCATGATGTGGTGATCGGGGAGATCGGTCTGACGGGAGAGGTG
CGTGGCGTATCGCGCATCGAACAGCGCGTGCGGGAAGCGCATAAGCTCGGCTTCAAACGCGTAATCATCCCGGAGAAAAA
TATTCGCGGCCTGGATGCTCCTGACGATATTCAGGTGATCGGGGTCAGCAATATCGCAGATGCTTTGAACGAGGTGATAA
GGAGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(337-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3PV01

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.814

99.341

0.664
  radA Streptococcus pneumoniae Rx1

57.837

99.56

0.576
  radA Streptococcus pneumoniae D39

57.837

99.56

0.576
  radA Streptococcus pneumoniae R6

57.837

99.56

0.576
  radA Streptococcus pneumoniae TIGR4

57.837

99.56

0.576
  radA Streptococcus mitis NCTC 12261

57.837

99.56

0.576
  radA Streptococcus mitis SK321

57.837

99.56

0.576