Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   JK088_RS14505 Genome accession   NZ_CP085857
Coordinates   3034141..3034719 (-) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain LH214     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3029141..3039719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JK088_RS14480 (JK088_14490) aroB 3029431..3030531 (-) 1101 WP_017449465.1 3-dehydroquinate synthase -
  JK088_RS14485 (JK088_14495) aroK 3030563..3031081 (-) 519 WP_005381319.1 shikimate kinase AroK -
  JK088_RS14490 (JK088_14500) pilQ 3031270..3033018 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  JK088_RS14495 (JK088_14505) pilP 3033050..3033565 (-) 516 WP_017449464.1 pilus assembly protein PilP Machinery gene
  JK088_RS14500 (JK088_14510) pilO 3033555..3034148 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  JK088_RS14505 (JK088_14515) pilN 3034141..3034719 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  JK088_RS14510 (JK088_14520) pilM 3034703..3035719 (-) 1017 WP_017449463.1 type IV pilus biogenesis protein PilM Machinery gene
  JK088_RS14515 (JK088_14525) - 3035862..3038432 (+) 2571 WP_017449462.1 penicillin-binding protein 1A -
  JK088_RS14520 (JK088_14530) oxyR 3038488..3039396 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=620868 JK088_RS14505 WP_015297396.1 3034141..3034719(-) (pilN) [Vibrio parahaemolyticus strain LH214]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=620868 JK088_RS14505 WP_015297396.1 3034141..3034719(-) (pilN) [Vibrio parahaemolyticus strain LH214]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCTGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCACGGCTAGCAACCATGCTCGATAACATGGAACGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTTGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTTAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594