Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   G157_RS03165 Genome accession   NC_022347
Coordinates   635588..636532 (-) Length   314 a.a.
NCBI ID   WP_021033688.1    Uniprot ID   -
Organism   Campylobacter coli CVM N29710     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 625454..671416 635588..636532 within 0


Gene organization within MGE regions


Location: 625454..671416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G157_RS03115 (G157_03130) - 625454..627010 (-) 1557 WP_002787752.1 DNA polymerase III subunit gamma/tau -
  G157_RS03120 (G157_03135) rho 627013..628311 (-) 1299 WP_002780613.1 transcription termination factor Rho -
  G157_RS03125 (G157_03140) - 628424..630787 (+) 2364 WP_072213620.1 heavy metal translocating P-type ATPase -
  G157_RS03130 (G157_03145) - 630780..630980 (+) 201 WP_002777240.1 cbb3-type cytochrome oxidase assembly protein -
  G157_RS03135 (G157_03150) - 631010..631309 (-) 300 WP_002777238.1 c-type cytochrome -
  G157_RS03140 (G157_03155) - 631386..631949 (+) 564 WP_002777235.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  G157_RS03145 (G157_03160) rfaD 631946..632899 (+) 954 WP_002796912.1 ADP-glyceromanno-heptose 6-epimerase -
  G157_RS03150 (G157_03165) rfaE1 632892..634277 (+) 1386 WP_002844795.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  G157_RS03155 (G157_03170) gmhA 634274..634834 (+) 561 WP_002806601.1 D-sedoheptulose 7-phosphate isomerase -
  G157_RS03160 (G157_03175) - 634834..635595 (+) 762 WP_021033687.1 glycosyltransferase family 25 protein -
  G157_RS03165 (G157_03180) waaF 635588..636532 (-) 945 WP_021033688.1 lipopolysaccharide heptosyltransferase II Regulator
  G157_RS03170 (G157_03185) - 636592..637413 (+) 822 WP_002844798.1 glycosyltransferase family 2 protein -
  G157_RS03175 (G157_03190) - 637456..638823 (+) 1368 WP_002844799.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  G157_RS03180 (G157_03195) gmd 638824..639963 (+) 1140 WP_002844800.1 GDP-mannose 4,6-dehydratase -
  G157_RS03185 (G157_03200) - 639956..640894 (+) 939 WP_002844801.1 NAD-dependent epimerase/dehydratase family protein -
  G157_RS03190 (G157_03205) - 640887..642056 (+) 1170 WP_002844803.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  G157_RS03195 (G157_03210) - 642434..643453 (+) 1020 WP_002844805.1 glycosyltransferase family 10 domain-containing protein -
  G157_RS03200 (G157_03215) - 643467..644438 (+) 972 WP_002844807.1 alpha-1,2-fucosyltransferase -
  G157_RS03205 (G157_03220) rfbF 644428..645249 (+) 822 WP_021033689.1 glucose-1-phosphate cytidylyltransferase -
  G157_RS03210 (G157_03225) rfbG 645253..646326 (+) 1074 WP_002844810.1 CDP-glucose 4,6-dehydratase -
  G157_RS03215 (G157_03230) - 646343..648100 (+) 1758 WP_002844812.1 thiamine pyrophosphate-binding protein -
  G157_RS03220 (G157_03235) - 648104..649021 (+) 918 WP_002844814.1 NAD-dependent epimerase/dehydratase family protein -
  G157_RS03225 (G157_03240) rfbH 649024..650346 (+) 1323 WP_002844816.1 lipopolysaccharide biosynthesis protein RfbH -
  G157_RS03230 (G157_03245) - 650362..651435 (+) 1074 WP_002844818.1 glycosyltransferase family 2 protein -
  G157_RS03235 (G157_03250) - 651483..652694 (+) 1212 WP_002844820.1 glycosyltransferase family 8 protein -
  G157_RS03240 (G157_03255) - 652681..653745 (-) 1065 WP_002844822.1 glycosyltransferase family 4 protein -
  G157_RS03245 (G157_03260) - 653742..655289 (-) 1548 WP_002844824.1 glycosyltransferase family 2 protein -
  G157_RS03250 (G157_03265) - 655286..656173 (-) 888 WP_002844825.1 lipid A biosynthesis lauroyl acyltransferase -
  G157_RS03255 (G157_03270) waaC 656166..657194 (-) 1029 WP_002844827.1 lipopolysaccharide heptosyltransferase I -
  G157_RS03260 (G157_03275) - 657259..658050 (+) 792 WP_023362001.1 3'-5' exonuclease -
  G157_RS03265 (G157_03280) galE 658107..659093 (+) 987 WP_002844831.1 UDP-glucose 4-epimerase GalE -
  G157_RS03270 (G157_03285) - 659087..660787 (+) 1701 WP_002844833.1 ABC transporter ATP-binding protein -
  G157_RS03275 (G157_03290) - 660784..661860 (+) 1077 WP_002844835.1 glycosyltransferase -
  G157_RS03280 (G157_03295) - 661853..662782 (+) 930 WP_002844836.1 glycosyltransferase family 2 protein -
  G157_RS03285 (G157_03300) - 662770..663867 (+) 1098 WP_002844838.1 glycosyltransferase -
  G157_RS03290 (G157_03305) - 663871..666015 (+) 2145 WP_002844839.1 STT3 domain-containing protein -
  G157_RS03295 (G157_03310) pglA 666027..667157 (+) 1131 WP_002777209.1 N, N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase -
  G157_RS03300 (G157_03315) pglC 667150..667752 (+) 603 WP_002777206.1 undecaprenyl phosphate N,N'-diacetylbacillosamine 1-phosphate transferase -
  G157_RS03305 (G157_03320) pglD 667739..668326 (+) 588 WP_002780649.1 UDP-N-acetylbacillosamine N-acetyltransferase -
  G157_RS03310 (G157_03325) pglE 668481..669641 (+) 1161 WP_002777202.1 UDP-N-acetylbacillosamine transaminase -
  G157_RS03315 (G157_03330) pglF 669644..671416 (+) 1773 WP_002777199.1 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 36126.75 Da        Isoelectric Point: 10.3770

>NTDB_id=62003 G157_RS03165 WP_021033688.1 635588..636532(-) (waaF) [Campylobacter coli CVM N29710]
MKIFIHLPTWLGDAVMASPALYGVYHHFKNAEFILYGSFVSTALFKEFPNAKIIVENKKSRYKQTLSLRKKLGKIDLALS
FRSALSSKIILHILKAKKRYFFNKYNFKEEHQVLKYLYFIENSLDIKAHSKDLKLPFRLKFQNPIVLKNGKKILGLNPGA
SFGSAKRWDASYFAQVALNFSKTHEILIFGAGKAEQELCDEIFHILKEKNVKVKNLCNKTTIKMLCQNIIFCDIFITNDS
GPMHIAAAYKTKTIAIFGPTKFTQTSPWQNQNTKLVHLNLACMPCMQKTCPLKHHQCMKDLKPEKILEAIRNFT

Nucleotide


Download         Length: 945 bp        

>NTDB_id=62003 G157_RS03165 WP_021033688.1 635588..636532(-) (waaF) [Campylobacter coli CVM N29710]
ATGAAAATTTTTATCCATCTTCCCACTTGGCTAGGCGATGCAGTCATGGCCTCACCTGCTTTATATGGAGTTTATCATCA
TTTTAAAAATGCTGAATTTATCCTTTATGGATCTTTTGTATCTACAGCGCTTTTTAAGGAATTTCCTAATGCTAAAATCA
TTGTAGAAAATAAAAAATCACGCTACAAACAAACCCTTTCTTTACGCAAAAAACTTGGAAAAATCGATCTAGCCCTTTCT
TTTAGATCAGCTCTTTCCTCTAAGATCATTTTGCATATCCTTAAGGCAAAAAAAAGATATTTTTTTAACAAGTATAATTT
CAAAGAAGAACATCAGGTTTTAAAATATCTTTATTTTATAGAAAATTCACTAGATATCAAAGCTCATTCTAAGGATTTAA
AACTCCCTTTTAGATTAAAATTTCAAAATCCTATTGTTTTAAAAAATGGCAAAAAAATCCTAGGCTTAAATCCTGGAGCA
AGTTTTGGAAGTGCTAAAAGATGGGATGCGAGTTATTTTGCACAAGTTGCTTTAAATTTTAGCAAAACTCATGAAATTTT
GATTTTTGGAGCGGGCAAGGCAGAACAAGAACTTTGCGATGAAATTTTTCATATATTAAAAGAAAAAAATGTCAAGGTAA
AAAATCTTTGCAATAAAACCACCATCAAAATGCTTTGTCAAAATATCATTTTTTGTGATATCTTCATCACAAATGATAGT
GGCCCTATGCACATAGCTGCCGCTTATAAAACAAAAACCATAGCTATCTTTGGGCCTACTAAATTTACCCAAACCTCACC
TTGGCAAAATCAAAATACAAAATTAGTACATCTAAATTTAGCTTGTATGCCTTGCATGCAAAAAACCTGCCCTTTAAAAC
ATCATCAGTGTATGAAAGATTTAAAACCGGAAAAAATTTTAGAAGCAATCCGAAATTTTACTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(108-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

82.428

99.682

0.822

Multiple sequence alignment