Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LIS82_RS00560 Genome accession   NZ_CP085712
Coordinates   109005..110384 (+) Length   459 a.a.
NCBI ID   WP_053478289.1    Uniprot ID   A0A0Q3RB35
Organism   Cytobacillus solani strain F27     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104005..115384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS82_RS00540 (LIS82_00540) - 104277..104738 (+) 462 WP_056681711.1 CtsR family transcriptional regulator -
  LIS82_RS00545 (LIS82_00545) - 104764..105312 (+) 549 WP_056681714.1 UvrB/UvrC motif-containing protein -
  LIS82_RS00550 (LIS82_00550) - 105314..106381 (+) 1068 WP_056681715.1 protein arginine kinase -
  LIS82_RS00555 (LIS82_00555) clpC 106398..108848 (+) 2451 WP_056681720.1 ATP-dependent protease ATP-binding subunit ClpC -
  LIS82_RS00560 (LIS82_00560) radA 109005..110384 (+) 1380 WP_053478289.1 DNA repair protein RadA Machinery gene
  LIS82_RS00565 (LIS82_00565) disA 110388..111461 (+) 1074 WP_053478288.1 DNA integrity scanning diadenylate cyclase DisA -
  LIS82_RS00570 (LIS82_00570) - 111676..112758 (+) 1083 WP_053478287.1 PIN/TRAM domain-containing protein -
  LIS82_RS00575 (LIS82_00575) ispD 112780..113469 (+) 690 WP_056681723.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LIS82_RS00580 (LIS82_00580) ispF 113495..113974 (+) 480 WP_056681726.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49832.28 Da        Isoelectric Point: 7.4031

>NTDB_id=619465 LIS82_RS00560 WP_053478289.1 109005..110384(+) (radA) [Cytobacillus solani strain F27]
MAKRKTKFMCQECGYESPKWMGKCPGCGEWNKMIEEVEKPATSRRGAFAHSAGSTILSKATPINAIETVSEPRIYTDLNE
LNRVLGGGVVRGSLVLIGGDPGIGKSTLLLQVSSQLANKNHSVLYISGEESMRQTKLRADRLGVSSDNLLVYSETSLEEI
SRTIDQVNPSFVIIDSIQTIFHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDKPTKPTDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPSNNLGGWKGPKGVELIGVGSVSEALKATLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=619465 LIS82_RS00560 WP_053478289.1 109005..110384(+) (radA) [Cytobacillus solani strain F27]
ATGGCCAAAAGGAAAACAAAGTTTATGTGCCAGGAATGCGGGTATGAATCACCAAAGTGGATGGGGAAATGCCCGGGCTG
TGGCGAGTGGAATAAAATGATCGAAGAAGTAGAGAAGCCTGCTACCTCAAGAAGGGGAGCATTTGCCCATTCCGCAGGAT
CGACCATTCTCTCAAAGGCGACGCCAATAAACGCTATTGAGACGGTAAGTGAGCCAAGAATATATACAGATTTAAATGAA
CTTAATCGTGTACTTGGAGGAGGAGTAGTTAGAGGTTCTCTTGTGTTAATCGGCGGTGATCCTGGAATCGGTAAATCTAC
GCTTCTCCTGCAGGTTTCATCCCAACTTGCTAATAAAAACCACTCTGTTTTGTATATATCTGGAGAAGAATCCATGCGTC
AAACGAAATTAAGAGCGGACCGTTTGGGTGTTTCTTCAGATAATTTATTAGTCTACTCGGAGACAAGTCTTGAAGAAATC
AGCAGGACAATCGATCAAGTAAATCCAAGCTTTGTAATCATTGATTCAATTCAAACAATCTTTCATCCAGAGGTTACTTC
AGCACCTGGAAGTGTTTCTCAAGTAAGGGAGTGCACGGCTGAGTTAATGAGAATCGGGAAGACAAAAGGAATTGCCATTT
TCATAGTTGGGCATGTTACAAAGGAAGGCTCTATAGCCGGACCGAGACTATTGGAGCATATGGTTGATACTGTGCTTTAT
TTTGAGGGCGAACGCCACCATACATATCGTATTTTACGAGCAGTAAAAAACAGGTTTGGATCTACAAATGAAATGGGAAT
TTTCGAAATGAAGGAGTTTGGATTGGAAGAGGTAGAAAATCCGTCCGAAATTTTCCTAGAGGAAAGATCCCAAGGTGCAG
CAGGATCAACTGTTGTTGCATCGATGGAAGGAACACGTCCAGTTCTCGTGGAGATACAGGCGCTTATTTCCCCAACAAGC
TTTGGAAATCCAAGAAGAATGGCAACGGGAATTGACCATAACCGTGTGCCATTGCTAATGGCAGTACTAGAAAAAAGGGT
AGGGATGCTCCTGCAGAACCAAGATGCTTATTTAAAGGTAGCTGGTGGAGTAAAGCTAGATGAGCCTGCTATCGATTTAG
CTATTGCTGTCAGTATTGCATCTAGTTTTCGTGATAAGCCAACTAAACCTACGGATTGTATTATTGGAGAAGTTGGATTA
ACTGGTGAAGTCAGAAGGGTCTCAAGAATTGAGCAACGGGTTCAGGAAGCTGCAAAGCTTGGCTTTGAACGAGTCATTCT
ACCATCTAATAATCTTGGTGGCTGGAAGGGACCAAAAGGAGTAGAACTAATAGGGGTAGGTTCTGTTAGTGAAGCATTAA
AAGCGACATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-33)

AAA domain (AAA_25)

(72-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q3RB35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.957

100

0.771
  radA Streptococcus mitis NCTC 12261

61.894

98.911

0.612
  radA Streptococcus pneumoniae Rx1

61.233

98.911

0.606
  radA Streptococcus pneumoniae D39

61.233

98.911

0.606
  radA Streptococcus pneumoniae R6

61.233

98.911

0.606
  radA Streptococcus pneumoniae TIGR4

61.233

98.911

0.606
  radA Streptococcus mitis SK321

61.013

98.911

0.603