Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SIR_RS18325 Genome accession   NC_022246
Coordinates   1789360..1790775 (-) Length   471 a.a.
NCBI ID   WP_021003271.1    Uniprot ID   T1ZFN0
Organism   Streptococcus intermedius B196     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1784360..1795775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIR_RS18300 (SIR_1682) - 1784489..1785169 (-) 681 WP_021003268.1 rhomboid family intramembrane serine protease -
  SIR_RS18305 (SIR_1683) - 1785162..1785689 (-) 528 WP_021003269.1 5-formyltetrahydrofolate cyclo-ligase -
  SIR_RS19920 (SIR_1684) - 1785735..1786865 (-) 1131 WP_009569532.1 N-acetyldiaminopimelate deacetylase -
  SIR_RS19925 (SIR_1685) dapD 1786878..1787576 (-) 699 WP_003075982.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase -
  SIR_RS18320 (SIR_1686) gltX 1787694..1789151 (-) 1458 WP_003073944.1 glutamate--tRNA ligase -
  SIR_RS18325 (SIR_1687) radA 1789360..1790775 (-) 1416 WP_021003271.1 DNA repair protein RadA Machinery gene
  SIR_RS18330 (SIR_1688) - 1790733..1791203 (-) 471 WP_021003272.1 cysteine hydrolase family protein -
  SIR_RS18335 (SIR_1689) - 1791213..1791656 (-) 444 WP_003073939.1 dUTP diphosphatase -
  SIR_RS18340 (SIR_1690) - 1791750..1792784 (-) 1035 WP_021003273.1 S66 peptidase family protein -
  SIR_RS18350 (SIR_1691) - 1793848..1794864 (+) 1017 WP_003073935.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -

Sequence

Protein


Download         Length: 471 a.a.        Molecular weight: 51677.23 Da        Isoelectric Point: 6.7134

>NTDB_id=61918 SIR_RS18325 WP_021003271.1 1789360..1790775(-) (radA) [Streptococcus intermedius B196]
MADCWNSNERRITIAKKKTTFVCQECGYNSPKYLGRCPNCSSWSSFVEEIEVAEVKNARVSLTGEKTRPVKLNQVTSSQA
TRVKTNMEEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVFYVSGEESAEQIKLRSERLGDIDNEFYLY
AETNMQSIRAEIEKIQPDFLIIDSIQTVMSPEISSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLE
HMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMRSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGTRPILAEV
QALVTPTVFGNAKRTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVRLDEPAIDLAVAVAIASSYKEKPTNPQE
CYIGEIGLTGEIRRVNRIEQRLNEASKLGFTKIFVPKNSLSGLTIPQGIQVVGVTTVGEVLKKVFDEVNKN

Nucleotide


Download         Length: 1416 bp        

>NTDB_id=61918 SIR_RS18325 WP_021003271.1 1789360..1790775(-) (radA) [Streptococcus intermedius B196]
ATGGCAGATTGCTGGAATAGTAACGAAAGGAGAATTACCATAGCTAAGAAAAAAACAACTTTTGTCTGTCAAGAGTGCGG
CTATAATTCGCCCAAGTATCTGGGACGTTGTCCTAATTGCTCGTCTTGGTCTTCTTTTGTTGAAGAGATTGAAGTTGCTG
AGGTTAAAAATGCTCGCGTTTCACTGACAGGTGAGAAGACACGGCCAGTCAAGTTAAATCAGGTCACCTCCAGCCAAGCC
ACTAGAGTCAAGACCAATATGGAAGAATTCAACCGTGTTTTGGGCGGTGGTGTTGTACCAGGCAGTCTGGTTCTTATCGG
CGGCGATCCCGGTATCGGGAAATCGACCCTACTTTTGCAAGTTTCAACACAATTAGCCAATAAAGGAACTGTTTTTTATG
TTTCTGGTGAAGAATCAGCAGAGCAGATCAAGTTGCGTAGCGAGCGTCTTGGTGATATTGACAATGAGTTTTATCTCTAT
GCTGAAACTAATATGCAAAGTATTCGTGCTGAGATTGAAAAAATCCAGCCTGATTTTTTAATTATTGATTCTATTCAAAC
AGTGATGAGTCCTGAAATTTCCAGTGTTCAAGGTTCTGTTTCTCAGGTGCGTGAAGTGACAGCAGAATTAATGCAATTAG
CTAAAACCAATAATATTGCGACTTTTATTGTTGGACATGTGACTAAAGAAGGAACCTTAGCTGGACCGCGAATGTTAGAA
CACATGGTGGACACTGTGCTCTACTTTGAAGGCGAGCGGCAGCATACTTTTCGTATCTTACGAGCCGTTAAAAATCGCTT
TGGTTCTACTAATGAGATTGGTATTTTTGAAATGAGGTCGGGTGGTTTGGTTGAGGTTTTGAATCCCAGCCAAGTTTTTT
TAGAAGAGCGTCTAGATGGAGCAACAGGCTCAGCTATTGTTGTTACCATGGAAGGAACACGGCCTATTTTAGCGGAGGTA
CAAGCCCTAGTAACACCGACTGTCTTTGGTAATGCTAAACGGACAACAACAGGACTGGACTTTAATCGTGTTAGTTTGAT
TATGGCGGTTTTGGAGAAGCGCTGCGGCCTTTTATTGCAAAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTGCGCT
TAGATGAGCCTGCTATTGATTTAGCGGTTGCGGTAGCCATTGCTTCCAGTTATAAGGAAAAGCCAACCAACCCTCAAGAA
TGTTATATTGGTGAAATTGGTTTGACAGGTGAAATTCGTCGTGTTAATCGTATCGAACAACGTCTCAATGAAGCTAGTAA
GCTCGGTTTTACCAAGATTTTTGTACCCAAAAATTCCCTGTCAGGTTTAACGATTCCACAAGGTATTCAGGTAGTTGGTG
TGACAACTGTTGGTGAGGTTTTGAAAAAGGTGTTTGACGAAGTAAATAAAAACTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-231)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(21-48)

Subunit ChlI of Mg-chelatase (ChlI)

(375-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T1ZFN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.938

95.966

0.854
  radA Streptococcus pneumoniae D39

88.938

95.966

0.854
  radA Streptococcus pneumoniae R6

88.938

95.966

0.854
  radA Streptococcus pneumoniae TIGR4

88.938

95.966

0.854
  radA Streptococcus mitis SK321

88.938

95.966

0.854
  radA Streptococcus mitis NCTC 12261

88.717

95.966

0.851
  radA Bacillus subtilis subsp. subtilis str. 168

61.81

96.178

0.594

Multiple sequence alignment