Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LEO77_RS01680 Genome accession   NZ_CP085465
Coordinates   344661..346100 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain 71506     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339661..351100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO77_RS01650 (LEO77_01650) mlaF 340238..341038 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  LEO77_RS01655 (LEO77_01655) - 341246..342241 (+) 996 WP_005336342.1 KpsF/GutQ family sugar-phosphate isomerase -
  LEO77_RS01660 (LEO77_01660) kdsC 342241..342795 (+) 555 WP_227341812.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LEO77_RS01665 (LEO77_01665) lptC 342792..343352 (+) 561 WP_227341813.1 LPS export ABC transporter periplasmic protein LptC -
  LEO77_RS01670 (LEO77_01670) lptA 343339..343866 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  LEO77_RS01675 (LEO77_01675) lptB 343870..344595 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  LEO77_RS01680 (LEO77_01680) rpoN/rpoN1 344661..346100 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  LEO77_RS01685 (LEO77_01685) hpf 346121..346408 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  LEO77_RS01690 (LEO77_01690) ptsN 346411..346857 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LEO77_RS01695 (LEO77_01695) rapZ 346903..347769 (+) 867 WP_040068818.1 RNase adapter RapZ -
  LEO77_RS01700 (LEO77_01700) npr 347790..348062 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  LEO77_RS01705 (LEO77_01705) degS 348209..349333 (-) 1125 WP_216952221.1 outer membrane-stress sensor serine endopeptidase DegS -
  LEO77_RS01710 (LEO77_01710) - 349443..350804 (-) 1362 WP_216952220.1 Do family serine endopeptidase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=618172 LEO77_RS01680 WP_005336331.1 344661..346100(+) (rpoN/rpoN1) [Aeromonas veronii strain 71506]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=618172 LEO77_RS01680 WP_005336331.1 344661..346100(+) (rpoN/rpoN1) [Aeromonas veronii strain 71506]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAAGAGCAGATGCCG
GATGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGACTCACACCGTTTA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCAGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAGCTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATTTGATCCAGCTGCTGGAGCCGCGCCCCGGCAATGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTTGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACTTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491