Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIS78_RS22780 Genome accession   NZ_CP085442
Coordinates   4389302..4390354 (-) Length   350 a.a.
NCBI ID   WP_116073650.1    Uniprot ID   -
Organism   Priestia megaterium strain KF18     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4384302..4395354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS78_RS22740 (LIS78_22760) - 4385317..4386141 (+) 825 WP_195781864.1 YqhG family protein -
  LIS78_RS22745 (LIS78_22765) - 4386166..4386351 (-) 186 WP_013059170.1 YqzE family protein -
  LIS78_RS22750 (LIS78_22770) comGG 4386395..4386769 (-) 375 WP_195781863.1 competence type IV pilus minor pilin ComGG -
  LIS78_RS22755 (LIS78_22775) comGF 4386759..4387196 (-) 438 WP_195781862.1 competence type IV pilus minor pilin ComGF -
  LIS78_RS22760 (LIS78_22780) - 4387193..4387531 (-) 339 WP_195781861.1 type II secretion system protein -
  LIS78_RS22765 (LIS78_22785) comGD 4387515..4387997 (-) 483 WP_252284390.1 competence type IV pilus minor pilin ComGD -
  LIS78_RS22770 (LIS78_22790) comGC 4387939..4388250 (-) 312 WP_252284391.1 competence type IV pilus major pilin ComGC -
  LIS78_RS22775 (LIS78_22795) comGB 4388265..4389311 (-) 1047 WP_209150663.1 competence type IV pilus assembly protein ComGB -
  LIS78_RS22780 (LIS78_22800) comGA 4389302..4390354 (-) 1053 WP_116073650.1 competence type IV pilus ATPase ComGA Machinery gene
  LIS78_RS22785 (LIS78_22805) - 4390625..4391008 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LIS78_RS22790 (LIS78_22810) - 4391270..4391506 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  LIS78_RS22795 (LIS78_22815) - 4391544..4392176 (-) 633 WP_116073652.1 MBL fold metallo-hydrolase -
  LIS78_RS22800 (LIS78_22820) - 4392385..4392561 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  LIS78_RS22805 (LIS78_22825) - 4392597..4393499 (-) 903 WP_195781859.1 M14 family metallocarboxypeptidase -
  LIS78_RS22810 (LIS78_22830) - 4393611..4394579 (-) 969 WP_252284392.1 ROK family glucokinase -
  LIS78_RS22815 (LIS78_22835) - 4394582..4394788 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39610.99 Da        Isoelectric Point: 9.0586

>NTDB_id=618151 LIS78_RS22780 WP_116073650.1 4389302..4390354(-) (comGA) [Priestia megaterium strain KF18]
MTEIEQFATYVIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=618151 LIS78_RS22780 WP_116073650.1 4389302..4390354(-) (comGA) [Priestia megaterium strain KF18]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTGCA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGATCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGCGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGGA
AGCATCGTCAGTCTAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATTGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586