Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIT34_RS23215 Genome accession   NZ_CP085431
Coordinates   4474874..4475926 (-) Length   350 a.a.
NCBI ID   WP_252281689.1    Uniprot ID   -
Organism   Priestia megaterium strain F20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4469874..4480926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT34_RS23175 (LIT34_23205) - 4470889..4471713 (+) 825 WP_013059169.1 YqhG family protein -
  LIT34_RS23180 (LIT34_23210) - 4471738..4471923 (-) 186 WP_013059170.1 YqzE family protein -
  LIT34_RS23185 (LIT34_23215) comGG 4471967..4472341 (-) 375 WP_013059171.1 competence type IV pilus minor pilin ComGG -
  LIT34_RS23190 (LIT34_23220) comGF 4472331..4472768 (-) 438 WP_013059172.1 competence type IV pilus minor pilin ComGF -
  LIT34_RS23195 (LIT34_23225) - 4472765..4473103 (-) 339 WP_252281688.1 type II secretion system protein -
  LIT34_RS23200 (LIT34_23230) comGD 4473087..4473524 (-) 438 WP_013059174.1 competence type IV pilus minor pilin ComGD -
  LIT34_RS23205 (LIT34_23235) comGC 4473511..4473822 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  LIT34_RS23210 (LIT34_23240) comGB 4473837..4474883 (-) 1047 WP_013059176.1 competence type IV pilus assembly protein ComGB -
  LIT34_RS23215 (LIT34_23245) comGA 4474874..4475926 (-) 1053 WP_252281689.1 competence type IV pilus ATPase ComGA Machinery gene
  LIT34_RS23220 (LIT34_23250) - 4476196..4476579 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LIT34_RS23225 (LIT34_23255) - 4476841..4477077 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  LIT34_RS23230 (LIT34_23260) - 4477115..4477747 (-) 633 WP_013059180.1 MBL fold metallo-hydrolase -
  LIT34_RS23235 (LIT34_23265) - 4477956..4478132 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  LIT34_RS23240 (LIT34_23270) - 4478168..4479070 (-) 903 WP_013059182.1 M14 family metallocarboxypeptidase -
  LIT34_RS23245 (LIT34_23275) - 4479181..4480149 (-) 969 WP_013059183.1 ROK family glucokinase -
  LIT34_RS23250 (LIT34_23280) - 4480152..4480358 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39754.18 Da        Isoelectric Point: 9.0586

>NTDB_id=618096 LIT34_RS23215 WP_252281689.1 4474874..4475926(-) (comGA) [Priestia megaterium strain F20]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFWVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=618096 LIT34_RS23215 WP_252281689.1 4474874..4475926(-) (comGA) [Priestia megaterium strain F20]
TTGACCGAAATTGAACAGTTTGCTACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACCCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACACACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGGTCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCACTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTTGGGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGAA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGTGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTGATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.286

100

0.583