Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LIT34_RS16855 Genome accession   NZ_CP085431
Coordinates   3304380..3304802 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain F20     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3299380..3309802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT34_RS16830 (LIT34_16860) - 3299619..3300440 (+) 822 WP_053487481.1 histidine kinase -
  LIT34_RS16835 (LIT34_16865) - 3300441..3301082 (+) 642 WP_053487482.1 response regulator transcription factor -
  LIT34_RS16840 (LIT34_16870) - 3301258..3302250 (+) 993 WP_063672078.1 glycerophosphodiester phosphodiesterase family protein -
  LIT34_RS16845 (LIT34_16875) - 3302363..3303745 (+) 1383 WP_082522657.1 PepSY domain-containing protein -
  LIT34_RS16850 (LIT34_16880) - 3303775..3304239 (+) 465 WP_252281580.1 FixH family protein -
  LIT34_RS16855 (LIT34_16885) nucA/comI 3304380..3304802 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LIT34_RS16860 (LIT34_16890) - 3304956..3305309 (+) 354 WP_223272376.1 DUF1516 family protein -
  LIT34_RS16865 (LIT34_16895) - 3305350..3305943 (-) 594 WP_013057951.1 hypothetical protein -
  LIT34_RS16870 (LIT34_16900) - 3306099..3306755 (-) 657 WP_252281581.1 TetR/AcrR family transcriptional regulator -
  LIT34_RS16875 (LIT34_16905) - 3306779..3308059 (-) 1281 WP_252281582.1 glycosyltransferase -
  LIT34_RS16880 (LIT34_16910) - 3308358..3308903 (+) 546 WP_013057954.1 GNAT family N-acetyltransferase -
  LIT34_RS16885 (LIT34_16915) - 3309203..3309688 (-) 486 WP_053487488.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=618085 LIT34_RS16855 WP_013057949.1 3304380..3304802(+) (nucA/comI) [Priestia megaterium strain F20]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=618085 LIT34_RS16855 WP_013057949.1 3304380..3304802(+) (nucA/comI) [Priestia megaterium strain F20]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCCGATAT
TGAATATGTAAAGCCTTCAGACAATCGAGGCGCCGGGTCTTGGATCTCACATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507