Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LIT30_RS19470 Genome accession   NZ_CP085426
Coordinates   3710472..3710846 (+) Length   124 a.a.
NCBI ID   WP_234014997.1    Uniprot ID   -
Organism   Priestia megaterium strain F16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3705472..3715846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT30_RS19450 (LIT30_19460) - 3705963..3706634 (-) 672 WP_252298603.1 response regulator transcription factor -
  LIT30_RS19455 (LIT30_19465) - 3706960..3707778 (+) 819 WP_013058559.1 phytoene/squalene synthase family protein -
  LIT30_RS19460 (LIT30_19470) - 3707814..3709763 (-) 1950 WP_053487648.1 FUSC family protein -
  LIT30_RS19465 (LIT30_19475) - 3709919..3710179 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  LIT30_RS19470 (LIT30_19480) nucA/comI 3710472..3710846 (+) 375 WP_234014997.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LIT30_RS19475 (LIT30_19485) comJ 3710882..3711289 (+) 408 WP_252298604.1 competence protein ComJ -
  LIT30_RS19480 (LIT30_19490) helD 3711318..3713636 (-) 2319 WP_013058564.1 RNA polymerase recycling motor HelD -
  LIT30_RS29610 - 3713881..3714006 (-) 126 WP_016765178.1 hypothetical protein -
  LIT30_RS19485 (LIT30_19495) - 3714146..3714664 (-) 519 WP_223272391.1 enoyl-CoA hydratase/isomerase family protein -
  LIT30_RS19490 (LIT30_19500) - 3714904..3715272 (+) 369 WP_029323759.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13631.88 Da        Isoelectric Point: 4.2601

>NTDB_id=618032 LIT30_RS19470 WP_234014997.1 3710472..3710846(+) (nucA/comI) [Priestia megaterium strain F16]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKAGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=618032 LIT30_RS19470 WP_234014997.1 3710472..3710846(+) (nucA/comI) [Priestia megaterium strain F16]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTATGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAGCAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645