Detailed information NTG0617908

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: LIS80_RS05975
• Genome accession: NZ_CP085413
• Coordinates: 1182166..1185891 (+)
• Length: 1241 a.a.
• NCBI ID: WP_000572276.1
• Uniprot ID: A0AAJ1LQQ5
• Organism: Bacillus paranthracis strain KF11
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1177166..1190891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIS80_RS05960 (LIS80_05960)	-	1177324..1177917 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
LIS80_RS05965 (LIS80_05965)	lepB	1177974..1178537 (+)	564	WP_000751900.1	signal peptidase I	-
LIS80_RS05970 (LIS80_05970)	addB	1178654..1182169 (+)	3516	WP_000058550.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
LIS80_RS05975 (LIS80_05975)	addA	1182166..1185891 (+)	3726	WP_000572276.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
LIS80_RS05980 (LIS80_05980)	-	1185904..1186191 (+)	288	WP_000179956.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
LIS80_RS05985 (LIS80_05985)	gerPF	1186224..1186439 (-)	216	WP_001141566.1	spore germination protein GerPF	-
LIS80_RS05990 (LIS80_05990)	-	1186482..1186868 (-)	387	WP_000902334.1	spore germination protein GerPE	-
LIS80_RS05995 (LIS80_05995)	gerPD	1186884..1187078 (-)	195	WP_001052802.1	spore germination protein GerPD	-
LIS80_RS06000 (LIS80_06000)	gerPC	1187085..1187699 (-)	615	WP_001070747.1	spore germination protein GerPC	-
LIS80_RS06005 (LIS80_06005)	gerPB	1187767..1187973 (-)	207	WP_001012508.1	spore germination protein GerPB	-
LIS80_RS06010 (LIS80_06010)	gerPA	1187988..1188209 (-)	222	WP_001111188.1	spore germination protein GerPA	-
LIS80_RS06015 (LIS80_06015)	-	1188306..1188485 (-)	180	WP_000462840.1	aspartyl-phosphate phosphatase Spo0E family protein	-
LIS80_RS06020 (LIS80_06020)	-	1188730..1189632 (+)	903	WP_000945297.1	fumarylacetoacetate hydrolase family protein	-
LIS80_RS06025 (LIS80_06025)	-	1189673..1189957 (-)	285	WP_000926868.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142759.00 Da
• Isoelectric Point: 4.8243

>NTDB_id=617908 LIS80_RS05975 WP_000572276.1 1182166..1185891(+) (addA) [Bacillus paranthracis strain KF11]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEEGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSVPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGQSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=617908 LIS80_RS05975 WP_000572276.1 1182166..1185891(+) (addA) [Bacillus paranthracis strain KF11]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCGGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTCATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACCGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGGATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGGAAAGCAACGGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCATGGACAAGCGTATATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGCGATTATAACGAGGATGTTGTGAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGCTATTTAGTCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGCGAACAAAGTGAAGAAGGTGAAATGAAGCCATCAGCTGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTTATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGGTTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGTTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCATGGGAAGAAAGGATCGTTTTATGAAGTAATGAGCTCATTTTTAAAAGGGGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTATTACAAGGATGGCGTGAATTTGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTTCCAGTCGTATTTGTCGCTGGACTTGGTCGTCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTTGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACAGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGAGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTGTTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTCGCGCCAGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAGGCCGTTCCATTAGAGAGTGAACGGAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGGACACGTCCTCGTTTTATGGAGAAAAAGGGATTAACATATGCAGAGCGCGG
AACAGCAGTACATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTCGAGCAGGCGGAAGAAATTGCGATTGAAAAAGTAATTTCATTCTTTGATAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGCAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGTGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.828	100	0.538