Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   LIT28_RS21900 Genome accession   NZ_CP085409
Coordinates   4260523..4261269 (-) Length   248 a.a.
NCBI ID   WP_063222930.1    Uniprot ID   A0A2B0XAC6
Organism   Bacillus thuringiensis strain KF1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4255523..4266269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT28_RS21885 (LIT28_21900) dnaG 4256291..4258087 (-) 1797 WP_199677512.1 DNA primase -
  LIT28_RS21890 (LIT28_21905) - 4258941..4259753 (-) 813 WP_060632300.1 pyruvate, water dikinase regulatory protein -
  LIT28_RS21895 (LIT28_21910) - 4259783..4260415 (-) 633 WP_000583758.1 helix-turn-helix transcriptional regulator -
  LIT28_RS21900 (LIT28_21915) recO 4260523..4261269 (-) 747 WP_063222930.1 DNA repair protein RecO Machinery gene
  LIT28_RS21905 (LIT28_21920) - 4261303..4261446 (-) 144 WP_000883924.1 YqzL family protein -
  LIT28_RS21910 (LIT28_21925) era 4261622..4262527 (-) 906 WP_048531222.1 GTPase Era -
  LIT28_RS21915 (LIT28_21930) - 4262520..4262918 (-) 399 WP_001086294.1 cytidine deaminase -
  LIT28_RS21920 (LIT28_21935) - 4263018..4263371 (-) 354 WP_242252267.1 diacylglycerol kinase family protein -
  LIT28_RS21925 (LIT28_21940) ybeY 4263368..4263838 (-) 471 WP_242252268.1 rRNA maturation RNase YbeY -
  LIT28_RS21930 (LIT28_21945) - 4263835..4265979 (-) 2145 WP_063222928.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28655.18 Da        Isoelectric Point: 8.0540

>NTDB_id=617879 LIT28_RS21900 WP_063222930.1 4260523..4261269(-) (recO) [Bacillus thuringiensis strain KF1]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDSEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=617879 LIT28_RS21900 WP_063222930.1 4260523..4261269(-) (recO) [Bacillus thuringiensis strain KF1]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGCCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTCCTTATACAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTACATGTGTGAGGGTGTTGATTCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCCGGGAAGGCGGTTTTCTGTGCTCGCGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTACGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTGTTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B0XAC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.061

99.597

0.548