Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIT28_RS21635 Genome accession   NZ_CP085409
Coordinates   4207884..4208927 (-) Length   347 a.a.
NCBI ID   WP_242252231.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain KF1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4202884..4213927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT28_RS21590 (LIT28_21605) - 4203313..4203513 (-) 201 WP_000106080.1 YqzE family protein -
  LIT28_RS21595 (LIT28_21610) aroK 4203552..4204049 (-) 498 WP_242252225.1 shikimate kinase AroK -
  LIT28_RS21600 (LIT28_21615) - 4204168..4204818 (-) 651 WP_063222963.1 2OG-Fe(II) oxygenase -
  LIT28_RS21605 (LIT28_21620) comGG 4204993..4205364 (-) 372 WP_063222962.1 competence type IV pilus minor pilin ComGG -
  LIT28_RS21610 (LIT28_21625) comGF 4205361..4205831 (-) 471 WP_242252226.1 competence type IV pilus minor pilin ComGF -
  LIT28_RS21615 (LIT28_21630) comGE 4205801..4206103 (-) 303 WP_242252227.1 competence type IV pilus minor pilin ComGE -
  LIT28_RS21620 (LIT28_21635) comGD 4206096..4206551 (-) 456 WP_242252228.1 comG operon protein ComGD -
  LIT28_RS21625 (LIT28_21640) comGC 4206548..4206847 (-) 300 WP_063222959.1 comG operon protein ComGC -
  LIT28_RS21630 (LIT28_21645) comGB 4206860..4207891 (-) 1032 WP_242252230.1 competence type IV pilus assembly protein ComGB -
  LIT28_RS21635 (LIT28_21650) comGA 4207884..4208927 (-) 1044 WP_242252231.1 competence protein ComGA Machinery gene
  LIT28_RS21640 (LIT28_21655) - 4209133..4209828 (+) 696 WP_242252234.1 helix-turn-helix domain-containing protein -
  LIT28_RS21645 (LIT28_21660) - 4209954..4210196 (+) 243 WP_000440713.1 DUF2626 family protein -
  LIT28_RS21650 (LIT28_21665) - 4210304..4211698 (+) 1395 WP_001094341.1 L-cystine transporter -
  LIT28_RS21655 (LIT28_21670) - 4211778..4212169 (-) 392 Protein_4238 hypothetical protein -
  LIT28_RS21660 (LIT28_21675) - 4212257..4212472 (-) 216 WP_001008320.1 DUF3912 family protein -
  LIT28_RS21665 (LIT28_21680) - 4212725..4213036 (+) 312 WP_242252235.1 hypothetical protein -
  LIT28_RS21670 (LIT28_21685) - 4213073..4213561 (-) 489 WP_242252237.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39281.76 Da        Isoelectric Point: 8.7292

>NTDB_id=617878 LIT28_RS21635 WP_242252231.1 4207884..4208927(-) (comGA) [Bacillus thuringiensis strain KF1]
MNGIESFANTILKEACRVQASDLHIVPRQKDVAIQLRIGKDLMMKHCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLSYSHGLLVFTGPTGSGKTTTMYALLEV
MRKKKTRRIVTLEDPVEKRNDDLLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGVTRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYTHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=617878 LIT28_RS21635 WP_242252231.1 4207884..4208927(-) (comGA) [Bacillus thuringiensis strain KF1]
ATGAATGGAATTGAAAGTTTTGCGAATACGATTTTGAAAGAAGCGTGTAGGGTACAAGCGTCGGACTTACATATCGTGCC
GAGGCAGAAGGACGTGGCAATTCAACTACGTATAGGAAAAGATTTAATGATGAAACATTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGAGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTACTTACGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
CCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCAAAAAAATTACTCTCTTTTTTAA
GTTATTCACATGGGTTACTTGTATTTACTGGACCGACTGGTTCTGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATGAGAAAAAAGAAAACGCGCCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCGGGACTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTTG
GTGAAATCCGTGATGAAGAAACAGCGAAAATCGCAATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAAAGGGGCGATACTCAGGTTCATGGATTTTGGCGTAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACACGCATGAAACATTAGAATCTTCGATACGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.411

100

0.36