Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LIT28_RS20405 Genome accession   NZ_CP085409
Coordinates   4001154..4001588 (-) Length   144 a.a.
NCBI ID   WP_063223129.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain KF1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3996154..4006588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT28_RS20390 (LIT28_20400) - 3997164..3997745 (+) 582 WP_063223131.1 PadR family transcriptional regulator -
  LIT28_RS20395 (LIT28_20405) metE 3998402..4000690 (+) 2289 WP_063223130.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  LIT28_RS20400 (LIT28_20410) comJ 4000728..4001117 (-) 390 WP_060632097.1 competence protein ComJ -
  LIT28_RS20405 (LIT28_20415) nucA/comI 4001154..4001588 (-) 435 WP_063223129.1 DNA-entry nuclease Machinery gene
  LIT28_RS20410 (LIT28_20420) - 4001825..4002574 (+) 750 WP_098555055.1 DUF3967 domain-containing protein -
  LIT28_RS20415 (LIT28_20425) kinB 4002613..4003887 (-) 1275 WP_242252078.1 sporulation sensor histidine kinase KinB -
  LIT28_RS20420 (LIT28_20430) - 4004197..4004397 (+) 201 WP_000929285.1 hypothetical protein -
  LIT28_RS20425 (LIT28_20435) - 4004521..4005684 (+) 1164 WP_063223126.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=617875 LIT28_RS20405 WP_063223129.1 4001154..4001588(-) (nucA/comI) [Bacillus thuringiensis strain KF1]
MKQLKGIIISIIAILSIVVAVYEVLIPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=617875 LIT28_RS20405 WP_063223129.1 4001154..4001588(-) (nucA/comI) [Bacillus thuringiensis strain KF1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTATTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACCGGGA
AACATATTACGGATGCGATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTACGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGGAATAAGCTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

83.333

0.556