Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   LIT28_RS00020 Genome accession   NZ_CP085409
Coordinates   3012..4139 (+) Length   375 a.a.
NCBI ID   WP_242254042.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain KF1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT28_RS00005 (LIT28_00005) dnaA 1..1341 (+) 1341 WP_097951318.1 chromosomal replication initiator protein DnaA -
  LIT28_RS00010 (LIT28_00010) dnaN 1520..2659 (+) 1140 WP_063223999.1 DNA polymerase III subunit beta -
  LIT28_RS00015 (LIT28_00015) yaaA 2787..2999 (+) 213 WP_000821369.1 S4 domain-containing protein YaaA -
  LIT28_RS00020 (LIT28_00020) recF 3012..4139 (+) 1128 WP_242254042.1 DNA replication/repair protein RecF Machinery gene
  LIT28_RS00025 (LIT28_00025) gyrB 4178..6100 (+) 1923 WP_063224001.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  LIT28_RS00030 (LIT28_00030) gyrA 6189..8660 (+) 2472 WP_063224002.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43391.71 Da        Isoelectric Point: 6.9850

>NTDB_id=617848 LIT28_RS00020 WP_242254042.1 3012..4139(+) (recF) [Bacillus thuringiensis strain KF1]
MFIKEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDYGQIKGSLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGEMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIIYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNGKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDNYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=617848 LIT28_RS00020 WP_242254042.1 3012..4139(+) (recF) [Bacillus thuringiensis strain KF1]
TTGTTTATTAAAGAAATACAATTAAAAAACTATCGCAATTATGAAAAATTAGAGCTTTCCTTTGAGGATAAGGTCAATGT
AATTATTGGCGAAAATGCACAAGGGAAAACAAATTTGATGGAAGCTATTTACGTATTAGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGTGAGCTCATTCGCTGGGATGAAGATTATGGTCAAATCAAAGGTAGCTTGCAAAAAAGAAACAGTTCT
TTGTCTTTAGAGTTAAATATTTCTAAAAAAGGCAAAAAGGCAAAGTTAAATCAACTTGAACAACAAAAACTAAGCCAATA
CATCGGTGAAATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAACTTGGACAAATAGCACCCGTTTATTTGTATGAACTAAGTCAATATCAAAAGGTGCTCACGCAACGA
AATCACTTGCTTAAAAAAATGCAAGGGAATAGCAAAAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTCATTGA
ACATGGTACAAAAATATTGCGAAAACGTTTTGAGTTTTTGCACTTACTACAAGAATGGGCTGCACCAATCCATCGTGGGA
TTAGTAGAGGATTAGAGGAGTTAGAAATCATCTATAAACCAAGCGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCCGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATAGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATGGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTACTCAGAAGTTAAGGAATATCCAATTCTTTTATTAGATGATGTTTTATCA
GAGCTAGATAATTATCGTCAATCACATCTGTTAAATACAATTCAAGGTAAGGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTGAAAGAAGCAAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGCATAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

66.576

98.133

0.653