Detailed information NTG0617743

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LIS81_RS25525
• Genome accession: NZ_CP085399
• Coordinates: 4922981..4924330 (-)
• Length: 449 a.a.
• NCBI ID: WP_226640888.1
• Uniprot ID: -
• Organism: Bacillus tropicus strain CK18
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4917981..4929330

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIS81_RS25505 (LIS81_25520)	secA	4918305..4920812 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
LIS81_RS25510 (LIS81_25525)	raiA	4921087..4921629 (-)	543	WP_000671191.1	ribosome-associated translation inhibitor RaiA	-
LIS81_RS25515 (LIS81_25530)	cspC	4921953..4922150 (-)	198	WP_001990088.1	cold shock protein CspC	-
LIS81_RS25520 (LIS81_25535)	-	4922277..4922981 (-)	705	WP_226640889.1	ComF family protein	-
LIS81_RS25525 (LIS81_25540)	comFA	4922981..4924330 (-)	1350	WP_226640888.1	ATP-dependent helicase ComFA	Machinery gene
LIS81_RS25530 (LIS81_25545)	-	4924458..4925891 (-)	1434	WP_147776475.1	C40 family peptidase	-
LIS81_RS25535 (LIS81_25550)	-	4926040..4926354 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
LIS81_RS25540 (LIS81_25555)	-	4926528..4927370 (-)	843	WP_029440995.1	DegV family protein	-
LIS81_RS25545 (LIS81_25560)	-	4927610..4928245 (+)	636	WP_000926682.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51270.95 Da
• Isoelectric Point: 9.8509

>NTDB_id=617743 LIS81_RS25525 WP_226640888.1 4922981..4924330(-) (comFA) [Bacillus tropicus strain CK18]
MLDGKQLLLEELSSDLRRELSDLKKKGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECTVLVRGIHERKGERELNSLQWKGALTTGQELAAQGVIKAIQQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELAPRLQEVFPRINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMNLNKKYPIFL
FVPHVRYIEDVSLLLKGLDNRIDGVHAEDPMRKEKVTSFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTEVMVRAKKHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=617743 LIS81_RS25525 WP_226640888.1 4922981..4924330(-) (comFA) [Bacillus tropicus strain CK18]
ATGCTTGATGGGAAACAGTTACTATTAGAAGAACTCTCTTCAGACTTACGGAGAGAATTAAGTGATTTAAAAAAGAAGGG
AGAGGTTATATGTGTACAAGGTGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGTTGTGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGCATAACGATGGGGAGAGTTAGT
GAATGTACTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGCGAAAGGGAATTAAATTCGTTACAGTGGAAAGGTGCATT
GACTACTGGTCAGGAGTTGGCGGCGCAAGGTGTCATAAAAGCGATTCAGCAGAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGAGCTGGAAAAACAGAGATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGCGCCGAGATTACAAGAAGTGTTTCCACGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCGTTTC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCATATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTTTCGGGACGATATCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGCGTGTTACTACAATGGTTAAAAATGAACTTAAACAAAAAGTATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGATGTGAGCCTGTTATTGAAAGGATTGGATAATCGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGACATCCTTCAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGAGTAACCGTGAAGAACTTACAAGTGGCTGTGTTAGGAGCTGAAGAAGAAATATTTTCGGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCTTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGGT
GATGGTACGTGCGAAAAAACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.452	98.441	0.497
comFA	Latilactobacillus sakei subsp. sakei 23K	40.955	88.641	0.363