Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIS81_RS20925 Genome accession   NZ_CP085399
Coordinates   4071488..4072531 (-) Length   347 a.a.
NCBI ID   WP_001013210.1    Uniprot ID   A0A5C5AE28
Organism   Bacillus tropicus strain CK18     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4066488..4077531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS81_RS20880 (LIS81_20895) - 4066917..4067117 (-) 201 WP_000106081.1 YqzE family protein -
  LIS81_RS20885 (LIS81_20900) aroK 4067156..4067653 (-) 498 WP_252210762.1 shikimate kinase AroK -
  LIS81_RS20890 (LIS81_20905) - 4067772..4068422 (-) 651 WP_142391841.1 2OG-Fe(II) oxygenase -
  LIS81_RS20895 (LIS81_20910) comGG 4068598..4068969 (-) 372 WP_252210763.1 competence type IV pilus minor pilin ComGG -
  LIS81_RS20900 (LIS81_20915) comGF 4068966..4069436 (-) 471 WP_000923091.1 competence type IV pilus minor pilin ComGF -
  LIS81_RS20905 (LIS81_20920) comGE 4069406..4069708 (-) 303 WP_048550802.1 competence type IV pilus minor pilin ComGE -
  LIS81_RS20910 (LIS81_20925) comGD 4069701..4070156 (-) 456 WP_252210764.1 comG operon protein ComGD -
  LIS81_RS20915 (LIS81_20930) comGC 4070153..4070452 (-) 300 WP_001178695.1 comG operon protein ComGC -
  LIS81_RS20920 (LIS81_20935) comGB 4070464..4071495 (-) 1032 WP_000471905.1 comG operon protein ComGB -
  LIS81_RS20925 (LIS81_20940) comGA 4071488..4072531 (-) 1044 WP_001013210.1 competence protein ComGA Machinery gene
  LIS81_RS20930 (LIS81_20945) - 4072736..4073431 (+) 696 WP_000434132.1 helix-turn-helix domain-containing protein -
  LIS81_RS20935 (LIS81_20950) - 4073557..4073799 (+) 243 WP_000440713.1 DUF2626 family protein -
  LIS81_RS20940 (LIS81_20955) - 4073907..4075301 (+) 1395 WP_001094339.1 L-cystine transporter -
  LIS81_RS20945 (LIS81_20960) - 4075478..4075885 (-) 408 WP_242274917.1 hypothetical protein -
  LIS81_RS20950 (LIS81_20965) - 4075973..4076188 (-) 216 WP_001008320.1 DUF3912 family protein -
  LIS81_RS20955 (LIS81_20970) - 4076441..4076752 (+) 312 WP_001093243.1 hypothetical protein -
  LIS81_RS20960 (LIS81_20975) - 4076788..4077276 (-) 489 WP_042875547.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39304.72 Da        Isoelectric Point: 8.7292

>NTDB_id=617732 LIS81_RS20925 WP_001013210.1 4071488..4072531(-) (comGA) [Bacillus tropicus strain CK18]
MNGIESFANTILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRSDDLLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGTILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCQKKCSTVCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=617732 LIS81_RS20925 WP_001013210.1 4071488..4072531(-) (comGA) [Bacillus tropicus strain CK18]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGACAGAAGGATGTAGTGGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTACGTCTTTCCACGCTTCCAACTGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGGTTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGCCGCATCGTTACACTGGAGGATCCAGTTGAAAAAAGAAGTGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACACTGCAT
ACAAATGATGCGAGAGGGACGATACTCAGGTTCATGGATTTTGGCATAACGAGGCAAGAGATTGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTTGAATTAAAGTGTCCGTTTTGCCAAAAAAAGTGCTCAACTGTATGCAAATCAATGAGGC
AAGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAACACGAAACATTAGAATCTTCGATACGAAAAGGATACGCTTTAGGATTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5AE28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576
  pilB Vibrio campbellii strain DS40M4

36.261

100

0.369