Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LIS81_RS19710 Genome accession   NZ_CP085399
Coordinates   3865624..3866058 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus tropicus strain CK18     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3860624..3871058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS81_RS19695 (LIS81_19710) - 3861647..3862204 (+) 558 WP_000062074.1 PadR family transcriptional regulator -
  LIS81_RS19700 (LIS81_19715) metE 3862872..3865160 (+) 2289 WP_074569289.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  LIS81_RS19705 (LIS81_19720) comJ 3865198..3865587 (-) 390 WP_000424012.1 competence protein ComJ -
  LIS81_RS19710 (LIS81_19725) nucA/comI 3865624..3866058 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  LIS81_RS19715 (LIS81_19730) - 3866295..3867044 (+) 750 WP_196472502.1 DUF3967 domain-containing protein -
  LIS81_RS19720 (LIS81_19735) kinB 3867098..3868372 (-) 1275 WP_000420393.1 sporulation sensor histidine kinase KinB -
  LIS81_RS19725 (LIS81_19740) - 3868682..3868882 (+) 201 WP_000929285.1 hypothetical protein -
  LIS81_RS19730 (LIS81_19745) - 3869006..3870163 (+) 1158 WP_000434566.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=617729 LIS81_RS19710 WP_000811502.1 3865624..3866058(-) (nucA/comI) [Bacillus tropicus strain CK18]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=617729 LIS81_RS19710 WP_000811502.1 3865624..3866058(-) (nucA/comI) [Bacillus tropicus strain CK18]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCATATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTGTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCATTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACACATTGAATATATAAGTCCAGCAGATAACCGTGGAGCAGGGTCTTGGGTAGGAAATAAGCTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549