Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIT27_RS21105 Genome accession   NZ_CP085392
Coordinates   4102435..4103505 (-) Length   356 a.a.
NCBI ID   WP_009332934.1    Uniprot ID   -
Organism   Cytobacillus oceanisediminis strain CK22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4097435..4108505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT27_RS21060 (LIT27_21100) - 4097927..4098721 (+) 795 WP_019380178.1 YqhG family protein -
  LIT27_RS21065 (LIT27_21105) - 4098768..4098953 (-) 186 WP_009332926.1 YqzE family protein -
  LIT27_RS21070 (LIT27_21110) - 4099031..4099528 (-) 498 WP_009332927.1 shikimate kinase -
  LIT27_RS21075 (LIT27_21115) comGG 4099541..4099924 (-) 384 WP_019380177.1 competence type IV pilus minor pilin ComGG -
  LIT27_RS21080 (LIT27_21120) comGF 4099914..4100393 (-) 480 WP_226280632.1 competence type IV pilus minor pilin ComGF -
  LIT27_RS21085 (LIT27_21125) - 4100344..4100673 (-) 330 WP_009332930.1 hypothetical protein -
  LIT27_RS21090 (LIT27_21130) comGD 4100657..4101097 (-) 441 WP_019380176.1 competence type IV pilus minor pilin ComGD -
  LIT27_RS21095 (LIT27_21135) comGC 4101094..4101417 (-) 324 WP_019380175.1 competence type IV pilus major pilin ComGC -
  LIT27_RS21100 (LIT27_21140) comGB 4101417..4102448 (-) 1032 WP_019380174.1 competence type IV pilus assembly protein ComGB -
  LIT27_RS21105 (LIT27_21145) comGA 4102435..4103505 (-) 1071 WP_009332934.1 competence type IV pilus ATPase ComGA Machinery gene
  LIT27_RS21115 (LIT27_21155) - 4103967..4104320 (-) 354 WP_019380414.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LIT27_RS21120 (LIT27_21160) - 4104567..4105265 (+) 699 WP_252245289.1 helix-turn-helix domain-containing protein -
  LIT27_RS21125 (LIT27_21165) - 4105416..4105661 (+) 246 WP_009332938.1 DUF2626 domain-containing protein -
  LIT27_RS21130 (LIT27_21170) - 4105707..4106768 (-) 1062 WP_019380411.1 SAM-dependent methyltransferase -
  LIT27_RS21135 (LIT27_21175) - 4106975..4107610 (-) 636 WP_009332940.1 MBL fold metallo-hydrolase -
  LIT27_RS21140 (LIT27_21180) - 4107699..4108178 (+) 480 WP_009332941.1 hypothetical protein -
  LIT27_RS21145 (LIT27_21185) - 4108304..4108477 (+) 174 WP_009332942.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40159.46 Da        Isoelectric Point: 8.8134

>NTDB_id=617466 LIT27_RS21105 WP_009332934.1 4102435..4103505(-) (comGA) [Cytobacillus oceanisediminis strain CK22]
MSVIERMAERIIKDAVRSHASDIHIIPRRKDTLIQLRFGSQLTPRLYLPKEECDRLISHFKFTASMDIGEKRRPQSGAYS
LEVDGQMIGLRFSTLPSSHSESLVIRILPQQEQIPFFQISLFPDMTRKMLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFHRNVITLEDPIEKENDLVLQVQVNEKAGVSYSAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAQGAVYRLAEFGVNMMEIEQTLVAVTAQRLVELNCPYCEGECSPFCYGYGRWKRASVFELLAGRALNASIKKARGER
SLVKYRTLKEVITKGIALGYIKETEYSRWVHDNAET

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=617466 LIT27_RS21105 WP_009332934.1 4102435..4103505(-) (comGA) [Cytobacillus oceanisediminis strain CK22]
TTGAGTGTAATTGAAAGAATGGCAGAACGAATTATCAAAGACGCCGTCAGGAGCCATGCTTCCGACATCCACATTATTCC
CCGAAGAAAAGACACTCTAATTCAGCTCAGGTTCGGCAGCCAATTGACCCCCAGGCTTTATCTTCCTAAAGAAGAGTGCG
ACAGACTGATTTCTCATTTTAAATTTACCGCTTCGATGGACATTGGTGAAAAGAGACGGCCGCAAAGCGGTGCCTATTCT
CTTGAAGTAGATGGACAAATGATTGGACTGCGTTTTTCAACCCTCCCATCAAGCCACAGCGAAAGCCTCGTTATCAGAAT
TCTGCCCCAGCAGGAACAAATTCCTTTTTTTCAAATCAGCCTTTTTCCAGACATGACCAGAAAAATGCTGGCATTGCTGA
AACATGCTCATGGATTAATTATTTTCACAGGACCAACTGGAAGCGGAAAAACCACAACATTGTATTCTCTGCTGAATGAA
ACCTCTCATATGTTCCATCGCAATGTGATTACCCTGGAAGATCCGATTGAAAAAGAGAATGACTTGGTCCTTCAAGTTCA
AGTCAATGAGAAAGCGGGCGTATCTTATTCCGCAGGCTTGAAAGCAATCCTTCGCCATGACCCCGATATCATTATGGTGG
GTGAAATCAGGGATGCGGAAACTGCGAAGATTGCTGTTAGAGCAGCACTTACAGGTCACCTTGTCTTAAGCACGATGCAT
ACTCGGGATGCTCAGGGGGCTGTATATCGTTTAGCTGAGTTTGGGGTGAATATGATGGAAATTGAGCAGACACTAGTGGC
TGTCACGGCTCAGCGCCTTGTCGAATTGAATTGCCCTTATTGTGAAGGGGAATGTTCTCCATTCTGCTATGGATACGGCA
GGTGGAAAAGGGCAAGTGTGTTTGAGCTTCTTGCAGGAAGAGCGCTTAATGCCTCCATAAAGAAGGCGAGAGGCGAAAGA
AGCCTTGTTAAGTATCGCACACTTAAAGAGGTAATAACTAAAGGGATTGCTCTTGGATATATAAAGGAAACTGAGTACAG
CAGGTGGGTGCACGATAATGCGGAAACATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.25

98.876

0.556
  pilB Glaesserella parasuis strain SC1401

40

98.315

0.393