Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIS77_RS18510 Genome accession   NZ_CP085390
Coordinates   3636447..3637517 (-) Length   356 a.a.
NCBI ID   WP_048008264.1    Uniprot ID   A0AA46SIG2
Organism   Cytobacillus firmus strain CK19     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3631447..3642517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS77_RS18465 (LIS77_18490) - 3631941..3632735 (+) 795 WP_048008256.1 YqhG family protein -
  LIS77_RS18470 (LIS77_18495) - 3632779..3632964 (-) 186 WP_035329975.1 YqzE family protein -
  LIS77_RS18475 (LIS77_18500) - 3633043..3633540 (-) 498 WP_252219376.1 shikimate kinase -
  LIS77_RS18480 (LIS77_18505) comGG 3633553..3633936 (-) 384 WP_252219379.1 competence type IV pilus minor pilin ComGG -
  LIS77_RS18485 (LIS77_18510) comGF 3633926..3634342 (-) 417 WP_197247512.1 competence type IV pilus minor pilin ComGF -
  LIS77_RS18490 (LIS77_18515) - 3634356..3634685 (-) 330 WP_222497764.1 phosphatase -
  LIS77_RS18495 (LIS77_18520) comGD 3634669..3635109 (-) 441 WP_252219382.1 competence type IV pilus minor pilin ComGD -
  LIS77_RS18500 (LIS77_18525) comGC 3635106..3635429 (-) 324 WP_252219385.1 competence type IV pilus major pilin ComGC -
  LIS77_RS18505 (LIS77_18530) comGB 3635429..3636460 (-) 1032 WP_252219387.1 competence type IV pilus assembly protein ComGB -
  LIS77_RS18510 (LIS77_18535) comGA 3636447..3637517 (-) 1071 WP_048008264.1 competence type IV pilus ATPase ComGA Machinery gene
  LIS77_RS18520 (LIS77_18545) - 3637786..3638139 (-) 354 WP_048008265.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LIS77_RS18525 (LIS77_18550) - 3638386..3639084 (+) 699 WP_163143401.1 helix-turn-helix domain-containing protein -
  LIS77_RS18530 (LIS77_18555) - 3639238..3639483 (+) 246 WP_009332938.1 DUF2626 domain-containing protein -
  LIS77_RS18535 (LIS77_18560) - 3639518..3640588 (-) 1071 WP_222498092.1 SAM-dependent methyltransferase -
  LIS77_RS18540 (LIS77_18565) - 3640793..3641428 (-) 636 WP_252219389.1 MBL fold metallo-hydrolase -
  LIS77_RS18545 (LIS77_18570) - 3641516..3641995 (+) 480 WP_163143407.1 hypothetical protein -
  LIS77_RS18550 (LIS77_18575) - 3642121..3642294 (+) 174 WP_048008269.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40081.29 Da        Isoelectric Point: 8.3347

>NTDB_id=617412 LIS77_RS18510 WP_048008264.1 3636447..3637517(-) (comGA) [Cytobacillus firmus strain CK19]
MSIIERLAERIIKDAVRSHASDIHIIPRRKDTLIQLRFGSQLTPRLYLPKEECDRLISHFKFTASMDIGEKRRPQSGAYS
LEVDGQMIGLRFSTLPSSHSESLVIRILPQQEQIPFYQISLFPDMTRKMLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFHRNVITLEDPIEKENDLVLQVQVNEKAGVSYSAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAEGAVYRLAEFGVNVLEIEQTLVAVTAQRLVELNCPYCEKECSPFCYGYGRWKRASVFELLAGRALNASIKKARGES
SLVKYRTLKEVITKGIALGYIKETEYSRWVHDDGEA

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=617412 LIS77_RS18510 WP_048008264.1 3636447..3637517(-) (comGA) [Cytobacillus firmus strain CK19]
TTGAGTATAATTGAAAGATTGGCAGAACGAATTATTAAAGACGCGGTCAGAAGCCATGCTTCCGACATCCACATCATTCC
CCGAAGAAAAGACACACTCATTCAGCTGAGGTTCGGCAGCCAGTTGACTCCAAGGCTTTATCTTCCCAAAGAAGAGTGTG
ACAGATTGATTTCCCATTTTAAATTTACAGCATCAATGGATATTGGTGAAAAAAGAAGGCCGCAGAGCGGTGCATACTCA
CTTGAAGTAGACGGACAAATGATTGGCCTGCGCTTTTCAACCCTCCCATCAAGCCACAGCGAGAGCCTCGTTATCAGAAT
TCTTCCCCAGCAGGAACAAATTCCTTTTTACCAAATCAGCCTTTTTCCAGACATGACAAGAAAAATGCTGGCACTGCTGA
AGCATGCTCATGGTTTAATTATTTTCACTGGCCCGACCGGCAGCGGTAAAACCACAACTTTGTATTCCCTTCTGAATGAA
ACCTCCCATATGTTCCATCGCAATGTCATCACCCTGGAAGATCCCATTGAAAAAGAGAATGACTTAGTTCTTCAAGTCCA
AGTGAATGAGAAAGCTGGCGTGTCTTATTCTGCAGGCTTAAAAGCAATCCTTCGCCATGACCCGGATATCATTATGGTCG
GTGAAATCCGGGATGCGGAAACGGCGAAGATTGCAGTTAGAGCAGCTCTCACCGGTCACCTTGTTTTAAGCACCATGCAT
ACACGGGATGCCGAGGGGGCTGTATACCGTTTAGCTGAGTTTGGAGTGAACGTGCTGGAAATTGAGCAGACTTTGGTGGC
GGTCACCGCACAGCGCCTCGTCGAATTGAATTGTCCATATTGTGAAAAGGAATGCTCCCCATTCTGCTATGGATATGGCA
GGTGGAAAAGGGCGAGTGTGTTTGAGCTGCTTGCAGGAAGGGCGCTCAATGCCTCCATAAAGAAGGCAAGAGGGGAAAGC
AGCCTTGTTAAGTATCGAACACTGAAGGAAGTCATAACTAAAGGGATTGCTCTGGGCTATATAAAGGAAACGGAGTACAG
CAGGTGGGTGCATGACGATGGAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.337

100

0.553
  pilB Glaesserella parasuis strain SC1401

40

98.315

0.393