Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   LIS79_RS05375 Genome accession   NZ_CP085389
Coordinates   1061577..1065281 (+) Length   1234 a.a.
NCBI ID   WP_025207108.1    Uniprot ID   -
Organism   Bacillus altitudinis strain CMF5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1056577..1070281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS79_RS05360 (LIS79_05360) - 1056875..1057285 (+) 411 WP_008348626.1 DoxX family protein -
  LIS79_RS05365 (LIS79_05365) - 1057477..1057923 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  LIS79_RS05370 (LIS79_05370) addB 1058090..1061599 (+) 3510 WP_060832216.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LIS79_RS05375 (LIS79_05375) addA 1061577..1065281 (+) 3705 WP_025207108.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LIS79_RS05380 (LIS79_05380) sbcD 1065307..1066482 (+) 1176 WP_060832217.1 exonuclease subunit SbcD -
  LIS79_RS05385 (LIS79_05385) sbcC 1066479..1069874 (+) 3396 WP_060832218.1 exonuclease subunit SbcC -
  LIS79_RS05390 (LIS79_05390) - 1069892..1070191 (+) 300 WP_017360217.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142646.59 Da        Isoelectric Point: 5.0967

>NTDB_id=617331 LIS79_RS05375 WP_025207108.1 1061577..1065281(+) (addA) [Bacillus altitudinis strain CMF5]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDTQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFGTLYELLPNINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKSVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADSNETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTTEMLLVHLDQHDTESDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDLSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQASDL
LKEDMLLEKQKQDEVVQALMEGVPVEGYGDYEEEVKERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSVRKAR
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLEEAEAMLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=617331 LIS79_RS05375 WP_025207108.1 1061577..1065281(+) (addA) [Bacillus altitudinis strain CMF5]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTCATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGATCACTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAAGTTCTCCGTACCTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGGTCACATCCAGCTCCCGAACAATGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAATTACCCTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGTCAGGAACTGCTGGAACAAGCG
CTCACTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGGGACACTTTATGAGCTTTTGCCAAACATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCGGCTCTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGATCAGCATTTAAAAAGCTTGGCTGAAATGAAGTCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAGAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCTGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAGTACTATCAACAGCAA
TTTGAAGAAGTACTCGTGGATGAATACCAGGATACAAACCTTGTCCAAGAAACGATTTTAAAGCTTGTGTCTAAAGGGAA
ACATCCTGCCGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTTAAAGCAGACAGCAATGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGT
AGCCGGGCTGATGTATTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACAGTTGGAGAAATTGAATATGA
CGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGACGACAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAGCGATGAAGAACGAGAAGAGCTAGAGACGGTTCAATTTGAGGCGAGAGTGATTGCAAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAGCTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGATCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCAGGTTATTTCGAAGCTACAGAAGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCTGCTGTTTTAAGGTCACCTATTGTTCATTTAGACGAAAATGAGATGGCACTGATTAGAACCAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACGTGCAGAAAGCTGG
AGCGTTTCTTCCTTCAATTACGCAAATGGCGTGATTTATCTATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGCAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCAAAACAGTATGAAAAGGCTGCTTTTAGAGGGCTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTGTTTACTGCAGGACTTGGCAGGAACTTTAATATGATGGATCTAAATCAGTCCTACTTGCTCGA
TAAAGAACTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAACTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAAGAAAAATTA
TTTTTAGTCGGTTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAACGGAGACGACTGGCTCCT
CCCTGATTTTGAACGTTATCAAGCGAGAACATACTTAGATTTTATTGGCCCTGCTCTTATCCGTCATCAAGCGATGTCAT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTCTCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTAAAAGAAGACATGTTACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGGGTGCCTGTTGAAGG
ATATGGCGATTACGAGGAGGAAGTAAAAGAAAGGCTTTCATGGCGATATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCTGAGATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTTCTTCCGTTCGAAAAGCTCGT
GCGACATTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAACCATATGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAGAGGAAGCTGAGGCGATGTTAAAAAAACGCTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTAGAGCGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.855

100

0.67