Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   LIS75_RS18035 Genome accession   NZ_CP085388
Coordinates   3622170..3623315 (+) Length   381 a.a.
NCBI ID   WP_171463628.1    Uniprot ID   -
Organism   Bacillus velezensis strain CMF18     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3617170..3628315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS75_RS18015 (LIS75_18025) - 3617644..3618360 (-) 717 WP_003151075.1 Crp/Fnr family transcriptional regulator -
  LIS75_RS18020 (LIS75_18030) narK 3618458..3619648 (-) 1191 WP_095318313.1 nitrate transporter NarK -
  LIS75_RS18025 (LIS75_18035) argS 3619783..3621453 (-) 1671 WP_043020956.1 arginine--tRNA ligase -
  LIS75_RS18030 (LIS75_18040) - 3621450..3621878 (-) 429 WP_003151069.1 DUF1934 family protein -
  LIS75_RS18035 (LIS75_18045) rapF 3622170..3623315 (+) 1146 WP_171463628.1 tetratricopeptide repeat protein Regulator
  LIS75_RS18040 (LIS75_18050) - 3623299..3623418 (+) 120 WP_015387559.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  LIS75_RS18045 (LIS75_18055) - 3623617..3623820 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  LIS75_RS18050 (LIS75_18060) - 3623834..3624037 (+) 204 WP_003151062.1 hypothetical protein -
  LIS75_RS19835 - 3624072..3624258 (+) 187 Protein_3508 DUF4177 domain-containing protein -
  LIS75_RS18055 (LIS75_18065) - 3624446..3624727 (+) 282 WP_014306021.1 hypothetical protein -
  LIS75_RS18060 (LIS75_18070) - 3624771..3625283 (+) 513 WP_032859656.1 stage II sporulation protein M -
  LIS75_RS18065 (LIS75_18075) - 3625874..3626092 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  LIS75_RS18070 (LIS75_18080) speB 3626262..3627134 (-) 873 WP_003151054.1 agmatinase -
  LIS75_RS18075 (LIS75_18085) speE 3627194..3628024 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45569.76 Da        Isoelectric Point: 4.6057

>NTDB_id=617313 LIS75_RS18035 WP_171463628.1 3622170..3623315(+) (rapF) [Bacillus velezensis strain CMF18]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLVDIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=617313 LIS75_RS18035 WP_171463628.1 3622170..3623315(+) (rapF) [Bacillus velezensis strain CMF18]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTATTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGTGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCTTT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATTATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567